Pom1 kinase links division plane position to cell polarity by regulating Mid1p cortical distribution
Pom1 kinase links division plane position to cell polarity by regulating Mid1p cortical distribution
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DOI：
10.1242/jcs.03261
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发表时间：
2006-11-15
影响因子：
4
通讯作者：
Paoletti, Anne
中科院分区：
文献类型：
--
作者：
Celton-Morizur, Severine;Racine, Victor;Paoletti, Anne
在分裂酵母中，Mid1p是决定分裂平面位置的主要因素，它定义了一个皮质内侧隔室，在那里它在有丝分裂开始时招募肌球蛋白II来启动收缩环组装。Mid1p如何局限于内侧皮质尚不清楚。我们在这里报道了在一个表现出单极生长模式的pom1极性突变体中，Mid1p的分布向不生长的细胞末端扩展，使Mid1p定位从核位置解偶联。这是有丝分裂过程中收缩环移位的原因。相比之下，Mid1p的局部化在Bud6 Delta株中是正常的，表明Mid1p的错误分布不是单极生长的一般结果。我们得出结论，Pom1激酶作为Mid1p分布的负调控因子，将Mid1p从非生长末端排除在外，而Pom1非依赖性机制阻止了Mid1p与生长末端的联系。因此，我们的工作提供了证据，证明细胞极性调节剂影响Mid1p的分布，将分裂平面位置与细胞极性联系起来。
In fission yeast, Mid1p, a major determinant for division plane position, defines a medial cortical compartment where it recruits myosin II at the onset of mitosis to initiate contractile ring assembly. How Mid1p is restricted to the medial cortex is unknown. We report here that in a pom1 polarity mutant, which displays a monopolar growth pattern, Mid1p distribution expands towards the non-growing cell tip, uncoupling Mid1p localization from nuclear position. This accounts for the displacement of the contractile ring during mitosis. By contrast, Mid1p localization is normal in a bud6 Delta strain, indicating that Mid1p misdistribution is not a general consequence of monopolar growth. We conclude that Pom1 kinase acts as a negative regulator of Mid1p distribution, excluding Mid1p from non-growing ends, whereas a Pom1-independent mechanism prevents Mid1p association with growing ends. Our work therefore provides evidence that cell polarity regulators influence the distribution of Mid1p, linking division plane position to cell polarity.