Cerebrospinal fluid oligoclonal bands in the diagnosis of multiple sclerosis - Isoelectric focusing with IgG immunoblotting compared with high-resolution agarose gel electrophoresis and cerebrospinal fluid IgG index
Cerebrospinal fluid oligoclonal bands in the diagnosis of multiple sclerosis - Isoelectric focusing with IgG immunoblotting compared with high-resolution agarose gel electrophoresis and cerebrospinal fluid IgG index
复制标题
DOI：
10.1309/y5vff2uaw0rk5w63
复制
发表时间：
2003-11-01
影响因子：
3.5
通讯作者：
Katzmann, JA
中科院分区：
文献类型：
--
作者：
Fortini, AS;Sanders, EL;Katzmann, JA
多发性硬化症（MS）是一种中枢神经系统炎症性脱髓鞘疾病。我们比较了等电聚焦（IEF）结合 IgG 免疫印迹与高分辨率琼脂糖凝胶电泳检测 MS 疾病过程中鞘内 IgG 合成的诊断性能。在 20 名确诊 MS 患者中，IEF 和高分辨率琼脂糖凝胶电泳的灵敏度分别为 90% 和 60%。在 51 名没有 MS 证据的患者中，该方法的特异性分别为 94% 和 96%。在该测试人群中患病率为 15%，IEF 和高分辨率琼脂糖凝胶电泳的阳性预测值分别为 73% 和 73%，阴性预测值分别为 98% 和 93%。 IEF 方法提高了寡克隆显带测定的灵敏度和阴性预测值，IEF 凝胶更容易解释，并且 IEF 测定需要更小的脑脊液体积。
Multiple sclerosis (MS) is an inflaminatory demyelinating disease of the central nervous system. We compared the diagnostic performance of isoelectric focusing (IEF) combined with IgG immunoblotting to high-resolution agarose gel electrophoresis for the detection of intrathecal synthesis of IgG due to the MS disease process.In 20 patients with definite MS, IEF and high-resolution agarose gel electrophoresis had sensitivities of 90% and 60%, respectively. In the 51 patients with no evidence of MS, the methods had specificities of 94% and 96%, respectively. With a prevalence of 15% in this test population, IEF and high-resolution agarose gel electrophoresis had positive predictive values of 73% and 73% and negative predictive values of 98% and 93%, respectively.The IEF method improves the sensitivity and the negative predictive value of the oligoclonal-banding assay, the IEF gels are easier to interpret, and the IEF assay requires a smaller cerebrospinal fluid volume.