Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis
Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis
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DOI：
10.1007/s00784-011-0654-4
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发表时间：
2012-12-01
影响因子：
3.4
通讯作者：
Chen, Hui
中科院分区：
文献类型：
--
作者：
He, Jiayan;Huang, Wujing;Chen, Hui
目的 本研究的目的是利用实时定量聚合酶链反应（qRT-PCR）确定中国人群中牙周病原菌的分布。 材料和方法 本横断面研究纳入了 24 名牙周健康的中国受试者和 60 名慢性牙周炎（CP）患者。采用 qRT-PCR 对 84 名受试者的唾液、龈上菌斑和龈下菌斑中收集的 252 个样本中的放线菌群、具核梭杆菌、牙龈卟啉单胞菌和中间普氏菌以及细菌总数进行定量。结果 伴放线菌的检出率低于 50%。所有受试者中均检测到具核梭菌，且脑瘫患者的细菌载量高于健康受试者。龈下菌斑中具核梭菌的中位比例显着高于龈上菌斑和唾液。与健康受试者相比，CP 患者中牙龈卟啉单胞菌和中间卟啉菌的检出频率和细菌载量更高。 CP组口腔内三个部位牙龈卟啉单胞菌的中位比例差异显着，其在龈下菌斑中的比例为9.01%。此外，三个口腔内位置的 A. actinomycetemcomitans、P. gingivalis 和 P. intermedia 计数均发现强正 Spearman 相关性。 结论 中国人群中这四种细菌的存在和细菌载量与其他人群相似。 临床相关性 检查中国成人中的细菌检测频率和载量可能有助于牙周病的微生物研究，并将为中国牙周病的诊断和抗生素治疗提供启示。人口。
Objective The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR).Materials and methods Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia as well as total bacterial counts from 252 samples collected from the saliva, supragingival plaque, and subgingival plaque of all 84 subjects.Results The detection frequency of A. actinomycetemcomitans was less than 50%. F. nucleatum was detected in all subjects and CP patients had higher bacterial loads than healthy subjects. The median proportion of F. nucleatum was significantly higher in subgingival plaque than in supragingival plaque and saliva. P. gingivalis and P. intermedia had higher detection frequencies and bacterial loads in CP patients than in healthy subjects. The median proportion of P. gingivalis was significantly different among the three intraoral locations in the CP group and its proportion in subgingival plaque was 9.01%. Moreover, strong positive Spearman's correlations were found in A. actinomycetemcomitans, P. gingivalis, and P. intermedia counts across the three intraoral locations.Conclusion The presence and bacteria loads of these four bacteria in this Chinese population are similar to those from other populations.Clinical relevance Examination of bacterial detection frequency and loads in Chinese adults may assist microbial studies of periodontal disease and will shed light on periodontal disease diagnosis and treatment using antibiotics in the Chinese population.