Osa-miR162a Enhances the Resistance to the Brown Planthopper via α-Linolenic Acid Metabolism in Rice (Oryza sativa)
Osa-miR162a Enhances the Resistance to the Brown Planthopper via α-Linolenic Acid Metabolism in Rice (Oryza sativa)
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DOI：
10.1021/acs.jafc.3c02637
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发表时间：
2023-07-26
影响因子：
6.1
通讯作者：
Zhang, Zhenfei
中科院分区：
文献类型：
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作者：
Chen, Jie;Liu, Qin;Zhang, Zhenfei
褐飞虱是造成水稻产量损失最严重的害虫。microRNAs（miRNAs）是植物-害虫相互作用的重要调控因子。在本研究中，我们发现osa-miR 162在幼苗期诱导响应BPH的攻击，并且通过气相色谱/液相色谱-质谱分析表明，水稻通过α-亚麻酸代谢途径对BPH产生抗性。过量表达osa-miR 162 a抑制了褐飞虱的生长发育，同时减少了3-己烯醛和3-己烯醇的释放，阻断了褐飞虱对寄主的识别;敲除osa-miR 162 a靶基因OsDCL 1，抑制了α-亚麻酸代谢，增强了褐飞虱的抗性，这与miR 162 a过量表达植株的结果相似。我们的研究揭示了在植物与寄主相互作用的长期进化过程中形成的植物miRNAs介导的一种新的防御机制，为水稻抗性资源的鉴定提供了新思路，并促进了对害虫防治的更好理解。
The brown planthopper (BPH) is the most serious pestcausing yieldlosses in rice. MicroRNAs (miRNAs) are emerging as key modulatorsof plant-pest interactions. In the study, we found that osa-miR162ais induced in response to BPH attack in the seedling stage and tunesrice resistance to the BPH via the & alpha;-linolenic acid metabolismpathway as indicated by gas chromatography/liquid chromatography-massspectrometry analysis. Overexpression of osa-miR162a inhibited thedevelopment and growth of the BPH and simultaneously reduced the releaseof 3-hexenal and 3-hexen-1-ol to block host recognition in the BPH.Moreover, knockdown of OsDCL1, which is targetedby osa-miR162a, inhibited & alpha;-linolenic acid metabolism to enhancethe resistance to the BPH, which was similar to that in miR162a-overexpressingplants. Our study revealed a novel defense mechanism mediated by plantmiRNAs developed during the long-term evolution of plant-hostinteraction, provided new ideas for the identification of rice resistanceresources, and promoted a better understanding of pest control.