APPROACH TO SINGLE-MOLECULE DETECTION USING SURFACE-ENHANCED RESONANCE RAMAN-SCATTERING (SERRS) - A STUDY USING RHODAMINE 6G ON COLLOIDAL SILVER
APPROACH TO SINGLE-MOLECULE DETECTION USING SURFACE-ENHANCED RESONANCE RAMAN-SCATTERING (SERRS) - A STUDY USING RHODAMINE 6G ON COLLOIDAL SILVER
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DOI：
10.1366/0003702953964480
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发表时间：
1995-06-01
影响因子：
3.5
通讯作者：
FELD, MS
中科院分区：
文献类型：
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作者：
KNEIPP, K;WANG, Y;FELD, MS
我们测量了浓度低至8×10~(-16)M的罗丹明6G(R6G)在氯化钠离子活化的胶体银溶液中的表面增强共振拉曼散射(SERRS)光谱。用514.5 nm的Ar~+激光谱线作为激发源，用光纤探头和电荷耦合器件探测系统测量光谱。在我们的实验精度范围内，SERRS照片计数与R6G浓度的相关性在8×10(-11)到8×10(-14)M浓度范围内呈线性关系。用较小的散射体积进行的实验表明，少于100个R6G分子就足以产生信噪比合理的SERRS光谱，这些结果表明在某些情况下SERRS可以达到与荧光光谱相当的检测极限，同时提供了比荧光更高的结构特异性。讨论了将SerRS用于单分子检测的可能性。
We have measured surface-enhanced resonance Raman scattering (SERRS) spectra of rhodamine 6G (R6G) at concentrations as low as 8 x 10(-16) M in colloidal silver solution activated by NaCl ions. The spectra were measured with a fiber-optic probe using the 514.5-nm argon-ion laser line as excitation source and a charge-coupled-device (CCD) detection system. The correlation of SERRS photo counts and R6G concentration was found to be linear between 8 x 10(-11) and 8 x 10(-14) M concentrations within our experimental accuracy. Experiments conducted with small scattering volumes show that fewer than 100 R6G molecules are sufficient to give rise to a SERRS spectrum with reasonable signal-to-noise ratio, These results demonstrate that in certain cases SERRS can achieve detection limits comparable to those for fluorescence spectroscopy, and at the same time provides higher structural specificity than fluorescence. The possibilities of using SERRS for single molecule detection are discussed.