Quantitative high contrast X-ray microtomography for dental research
Quantitative high contrast X-ray microtomography for dental research
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DOI：
10.1016/j.jdent.2013.01.010
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发表时间：
2013-05-01
影响因子：
4.4
通讯作者：
Mills, David
中科院分区：
文献类型：
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作者：
Davis, Graham R.;Evershed, Anthony N. Z.;Mills, David
目的：X射线显微断层扫描（XMT或micro-CT）是医学CT的小型化版本，已广泛用于体外牙科研究。该技术允许三维分析的结构和密度（或浓度），后者需要一些先验知识的组成。本文的目的是证明专门为牙科研究设计的XMT扫描仪的能力，特别是需要高对比度分辨率或准确的矿物质浓度quantization.Methods：在玛丽皇后，伦敦大学设计的MuCAT扫描仪，使用高动态范围的CCD相机与时间延迟积分读出，以提供高品质的断层图像。矿物浓度定量的准确性是通过使用建模方法来实现的，以进行射束硬化校正，并在每次扫描后进行自动校准。准确性进行了测试，通过测量的羟基磷灰石光盘的密度，和实用程序的高对比度和高精度的能力，证明在三个应用程序从正在进行的牙科research projects.Results：羟基磷灰石光盘密度的量化精度优于1%。在牙科应用中，矿物质浓度的轻微差异允许的功能，如死道可视化和离子运输从玻璃离子水泥到脱矿dentinesobserved.Conclusion：提高的准确性和对比敏感度，连同非破坏性的XMT一般，有利于牙齿的动态过程的精确研究。能够区分矿物质浓度的细微差异，可以在三维空间中追踪死道，将牙齿的外部病理与牙髓的反应过程联系起来。临床意义：MuCAT扫描仪经过优化，可用于矿物质浓度的量化，特别适用于脱矿和再矿化、挖掘和其他处理方法的体外研究，并进一步深入了解牙齿形态和病理学。此类研究的结果将为临床治疗和管理的发展提供信息。(C)2013爱思唯尔有限公司保留所有权利。
Objectives: X-ray microtomography (XMT or micro-CT) is a miniaturized version of medical CT and has been used extensively for in vitro dental research. The technique allows three-dimensional analyses of both structure and density (or concentration), the latter requiring some a priori knowledge of composition. The aim of this paper is to demonstrate the capabilities of an XMT scanner specifically designed for dental research, especially that requiring high contrast resolution or accurate mineral concentration quantification.Methods: The MuCAT scanners designed at Queen Mary, University of London, use high dynamic range CCD cameras with time-delay integration readout to provide high quality tomographic images. Accuracy in mineral concentration quantification is achieved using a modelling approach to beam hardening correction with automatic calibration following every scan. Accuracy was tested by measuring the density of a hydroxyapatite disc, and the utility of the high contrast and high accuracy capabilities are demonstrated in three applications from ongoing dental research projects.Results: Quantification accuracy in the hydroxyapatite disc density was better than 1%. In dental applications, slight differences in mineral concentration allowed features such as dead tracts to be visualized and ion transport from a glass-ionomer cement into demineralized dentine to be observed.Conclusion: The improved accuracy and contrast sensitivity, together with the non-destructive nature of XMT in general, facilitates precise studies of dynamic processes in teeth. The ability to differentiate subtle differences in mineral concentration allows dead tracts to be traced in three dimensions, linking external pathology in teeth to reactive processes from the pulp.Clinical significance: The MuCAT scanners have been optimized for quantification of mineral concentration and are particularly useful for in vitro studies of de-and re-mineralization, excavation and other treatment methodologies, and for gaining further insight into tooth morphology and pathology. Results from such studies will inform development of clinical treatment and management. (C) 2013 Elsevier Ltd. All rights reserved.