Selective killing of leukemic cells by merocyanine 540-mediated photosensitization.
Selective killing of leukemic cells by merocyanine 540-mediated photosensitization.
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通过部花青 540 介导的光敏作用选择性杀死白血病细胞。
DOI：
10.1073/pnas.81.23.7584
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发表时间：
1984
影响因子：
11.1
通讯作者：
Sutcliffe,AM
中科院分区：
文献类型：
--
作者：
Sieber,F;Spivak,JL;Sutcliffe,AM
白血病骨髓与亲脂性荧光染料mercury 540的体外孵育以及同时暴露于光下使L1210白血病细胞的浓度降低了约5个数量级，但保留了足够的正常多能造血干细胞以允许经致死性照射的同基因小鼠的造血重建。这种简单而快速的方法可能会在自体骨髓移植物中清除肿瘤细胞方面找到应用。
In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.