Target Recycling Transcription of Lighting-Up RNA Aptamers for Highly Sensitive and Label-Free Detection of ATP
Target Recycling Transcription of Lighting-Up RNA Aptamers for Highly Sensitive and Label-Free Detection of ATP
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DOI：
10.1007/s41664-021-00170-3
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发表时间：
2021-05
影响因子：
4.7
通讯作者：
Jialun He;Bingying Jiang;Wenjiao Zhou;R. Yuan;Yun Xiang
中科院分区：
文献类型：
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作者：
Jialun He;Bingying Jiang;Wenjiao Zhou;R. Yuan;Yun Xiang
我们在这里描述了一种靶循环转录的点亮适体策略，用于检测人类血清中ATP的无标记手段，具有高灵敏度。ATP分子特异性识别结合适配体，通过脚点介导的链位移反应，导致DNA组装探针的结构切换，模仿靶ATP分子的循环循环，从而形成许多含有RNA启动子序列的dsDNAs，用于随后转录产生大量的点亮适配体。然后，有机染料孔雀石绿与这些发光适配体结合产生显著增强的荧光信号，可以在10到500 nM的动态范围内以无标记的方式灵敏地检测ATP。该传感方法的检测限为7.3 nM，对ATP类似物分子也有很好的选择性。此外，该方法可以灵敏地检测稀释后的人血清样品中的ATP分子，这证明了在诊断ATP相关疾病方面的良好潜力。
We describe here a target recycling transcription of lighting-up aptamer strategy for detecting ATP in human serums in a label-free means with high sensitivity. ATP molecules specifically recognize the binding aptamer and result in the structure switching of the DNA assembly probes to imitate the target ATP molecule recycling cycles through the toehold-mediated strand displacement reaction, which causes the formation of many dsDNAs containing the RNA promoter sequences for subsequent transcription generation of large amounts of lighting-up aptamers. The organic dye, malachite green, then associates with these lighting-up aptamers to produce significantly enhanced fluorescence signals, which can sensitively detect ATP within a dynamic range from 10 to 500 nM in a label-free way. The sensing approach shows a detection limit of 7.3 nM and also has an excellent selectivity for ATP analogue molecules. In addition, this method can detect ATP molecules in diluted human serum samples sensitively, which proves the promising potential to diagnose ATP-related diseases.