Iris movement mediates vascular apoptosis during rat pupillary membrane regression.
Iris movement mediates vascular apoptosis during rat pupillary membrane regression.
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DOI：
10.1152/ajpregu.00602.2005
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发表时间：
2006-03
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通讯作者：
Y. Morizane;S. Mohri;J. Kosaka;S. Toné;T. Kiyooka;T. Miyasaka;J. Shimizu;Y. Ogasawara;F. Shiraga;Y. Minatogawa;J. Sasaki;H. Ohtsuki;F. Kajiya
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文献类型：
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作者：
Y. Morizane;S. Mohri;J. Kosaka;S. Toné;T. Kiyooka;T. Miyasaka;J. Shimizu;Y. Ogasawara;F. Shiraga;Y. Minatogawa;J. Sasaki;H. Ohtsuki;F. Kajiya
在哺乳动物透镜发育过程中，瞳孔区形成一层毛细血管网，即瞳孔膜（PM），PM覆盖透镜的前表面，然后后退，使光路通畅。虽然在PM回归中已经报道了凋亡过程的参与，但启动因素仍然未知。我们最初发现，PM的消退与虹膜运动的发展相一致，虹膜运动引起PM内血流的停止和恢复。因此，我们研究了虹膜收缩和扩张功能的发展是否是诱导PM细胞凋亡的重要信号。从出生后第7天到第12天连续用散瞳剂抑制虹膜运动抑制PM的凋亡和巨噬细胞向PM的迁移，并导致大鼠PM的持续存在。凋亡细胞在消退的PM中的分布是弥漫性的，并且没有表现出明显的定位。这些结果表明，虹膜运动通过改变其内部的血流来诱导PM的消退。本研究表明了组织之间的生理相互作用的重要性-在这种情况下，虹膜和PM-作为在器官发育期间促进血管消退的信号，并且除了众所周知的功能之外，还定义了虹膜在眼部发育期间的新功能，即，优化进入眼睛的光传输。
In the course of mammalian lens development, a transient capillary meshwork known as the pupillary membrane (PM) forms, which is located at the pupil area; the PM nourishes the anterior surface of the lens and then regresses to make the optical path clear. Although the involvement of apoptotic process has been reported in the PM regression, the initiating factor remains unknown. We initially found that regression of the PM coincided with the development of iris motility, and iris movement caused cessation and resumption of blood flow within the PM. Therefore, we investigated whether the development of the iris's ability to constrict and dilate functions as an essential signal that induces apoptosis in the PM. Continuous inhibition of iris movement with mydriatic agents from postnatal day 7 to day 12 suppressed apoptosis of the PM and migration of macrophage toward the PM, and resulted in the persistence of PM in rats. The distribution of apoptotic cells in the regressing PM was diffuse and showed no apparent localization. These results indicated that iris movement induced regression of the PM by changing the blood flow within it. This study suggests the importance of the physiological interactions between tissues-in this case, the iris and the PM-as a signal to advance vascular regression during organ development, and defines a novel function of the iris during ocular development in addition to the well-known function, that is, optimization of light transmission into the eye.