Comparative analysis identifies significant peptides related to asthma mechanism
Comparative analysis identifies significant peptides related to asthma mechanism
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比较分析确定与哮喘机制相关的重要肽
DOI：
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Xingyun Wang
中科院分区：
文献类型：
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作者：
Danying Zhu;Chao Wang;Chaoyue Meng;Yiwen Liu;Zeyu Zeng;Ran Zhao;Xiaoyan Dong;Xingyun Wang
哮喘是一种慢性炎症性变态反应性疾病。肽在哮喘治疗中显示出巨大的潜力。我们的研究旨在确定哮喘和非哮喘小鼠之间的差异肽组谱。方法和结果：用屋尘螨（HDM）建立哮喘小鼠模型。肺组织进行组织学分析和液相色谱-质谱（LC-MS/MS）检测。肺组织的组织学分析显示嗜酸性粒细胞浸润、支气管壁增厚、粘膜肿胀和充血。其中，1564个肽中的108个被鉴定并显示出显著的差异表达（倍数变化>2或倍数变化<0.5，P值<0.05），包含44个上调和64个下调的肽。GO分析表明，所鉴定的肽的功能性前体蛋白主要与肌动蛋白聚合或解聚、受体介导的内吞作用（RME）和炎症反应的调节相关。KEGG分析显示，这些肽与囊泡转运、上皮细胞的细菌侵袭和紧密连接信号通路中的可溶性N-乙基马来酰亚胺敏感性因子附着蛋白受体（SNARE）相互作用有关。前体蛋白分析显示，富含谷氨酸的蛋白样3（SH 3BGRL 3）衍生肽可能与哮喘的发病有关。结论：本研究结果为肽类药物治疗哮喘的候选部位提供了依据。
Asthma is a kind of chronic inflammatory and allergic disease. Peptides have showed significant potential .for asthma therapeutics. Our study aims to identify the differential peptidomic profiles between asthmatic and nonasthmatic mice. Methods and results: House dust mite (HDM) was utilized to build an asthmatic mouse model. Lung .tissues were tested by histological analysis and liquid chromatography-mass spectrometry (LC-MS/MS). Histological .analysis of lung tissues showed eosinophils infiltration, thickening of the bronchial wall, swelling, and hyperemia .of the mucosa. In which, 108 of 1564 peptides were identified and showed significant differential expression (fold .change >2 or fold change <0.5, P-value <0.05), containing 44 upregulated and 64 downregulated peptides. GO .analysis demonstrated that the functional precursor proteins of the identified peptides were primarily associated .with actin polymerization or depolymerization, receptor-mediated endocytosis (RME), and regulation of the inflammatory response. KEGG analysis revealed that the peptides were associated with soluble N-ethylmaleimide-sensitive .factor attachment protein receptor (SNARE) interactions in vesicular transport, bacterial invasion of epithelial cells, .and tight junction signaling pathways. Precursor proteins analysis revealed that peptides derived from glutamic .acid-rich protein-like 3 (SH3BGRL3) might be related to the incidence of asthma. Conclusions: Our results provide .evidence for the candidate treatment sites of peptides in asthma.