PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells
PEDOT:PSS organic electrochemical transistor arrays for extracellular electrophysiological sensing of cardiac cells
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DOI：
10.1016/j.bios.2016.09.047
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发表时间：
2017-07-15
影响因子：
12.6
通讯作者：
Ingebrandt, Sven
中科院分区：
文献类型：
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作者：
Hempel, Felix;Law, Jessica Ka-Yan;Ingebrandt, Sven
嵌入在平面器件中的电生理生物传感器代表了测量和评估生物系统电活动的最新方法。这种测量方法允许测试药物及其对细胞或组织的影响、细胞毒性以及直接实施到体内生物系统中进行信号转导。玻璃基板上具有金属或类金属电极的多电极阵列（MEA）是用于此目的的最常见、最成熟的平台之一。近年来，由聚（2，3-二氢噻吩并-1，4-二恶英）-聚（苯乙烯磺酸盐）（PEDOT：PSS）制成的有机电化学晶体管（OECTs）在生物体系中的离子信号转导和放大方面也显示出了其价值。我们在晶圆级工艺中开发了OECT器件，并将其用作测量心脏细胞系HL-1电生理活性的电生理生物传感器。我们优化的设备显示出非常有前途的性能，如良好的信噪比以及记录细胞外信号的快速成分的能力。结合简单，成本效益高的制造和聚合物的透明度，该平台为未来的细胞传感应用提供了传统MEA系统的有价值的替代方案。
Electrophysiological biosensors embedded in planar devices represent a state of the art approach to measure and evaluate the electrical activity of biological systems. This measurement method allows for the testing of drugs and their influences on cells or tissues, cytotoxicity, as well as the direct implementation into biological systems in vivo for signal transduction. Multi-electrode arrays (MEAs) with metal or metal-like electrodes on glass substrates are one of the most common, well-established platforms for this purpose. In recent years organic electrochemical transistors (OECTs) made of poly(2,3-dihydrothieno-1,4-dioxin)-poly(styrenesulfonate) (PEDOT:PSS) have as well shown their value in transducing and amplifying the ionic signals in biological systems. We developed OECT devices in a wafer-scale process and used them as electrophysiological biosensors measuring electrophysiological activity of the cardiac cell line HL-1. Our optimized devices show very promising properties such as good signal-to-noise ratio as well as the ability to record fast components of extracellular signals. Combined with an easy, cost effective fabrication and the transparency of the polymer, this platform offers a valuable alternative to traditional MEA systems for future cell sensing applications.