Ex Vivo Brain Imaging in Drosophila.
Ex Vivo Brain Imaging in Drosophila.
复制标题
果蝇离体脑成像。
DOI：
10.1101/pdb.prot108136
复制
发表时间：
2023
影响因子：
--
通讯作者：
Tomchik,SethM
中科院分区：
文献类型：
--
作者：
Boto,Tamara;Tomchik,SethM
通过离体成像制剂可以促进果蝇神经元回路功能的分析。在这种方法中，大脑是孤立的但完整的，保留了神经元的连接和功能。该制剂具有多种优点，包括稳定性、药理学操作的可及性以及能够在数小时内成像。果蝇中可用的全套遗传方法可以很容易地与该制剂中的药理学操作相结合，并且许多遗传编码的报告基因可用于对细胞事件进行成像，从 Ca 2+ 信号传导到神经递质释放。
Analysis of neuronal circuit function in Drosophila can be facilitated with an ex vivo imaging preparation. In this approach, the brain is isolated but intact, preserving neuronal connectivity and function. The preparation has several advantages, including stability, accessibility for pharmacological manipulations, and the ability to image over several hours. The full range of genetic approaches available in Drosophila can be readily combined with pharmacological manipulations in this preparation, and numerous genetically encoded reporters are available to image cellular events, ranging from Ca 2+ signaling to neurotransmitter release.