Pollen grains for oral vaccination.
Pollen grains for oral vaccination.
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DOI：
10.1016/j.jconrel.2014.08.010
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发表时间：
2014-11-28
期刊：
影响因子：
--
通讯作者：
Gill HS
中科院分区：
文献类型：
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作者：
Atwe SU;Ma Y;Gill HS
口服疫苗可以提供一种无痛和方便的疫苗接种方法。此外，除了全身免疫外，它还可能通过肠道相关淋巴组织的抗原加工刺激粘膜免疫。在这项研究中，我们提出的概念，花粉粒可以被设计为一个简单的模块化系统，用于口服疫苗。我们证明了这一概念的可行性，通过使用石松（石松）（LS）的孢子。我们表明，LS可以被化学清洗，以去除天然蛋白质，以创建完整的清洁中空LS壳。空的花粉壳成功地填充了不同大小的分子，证明了它们作为疫苗接种系统的广泛应用的潜力。使用卵清蛋白（OVA）作为模型抗原，将用OVA配制的LS经口饲喂给小鼠。LS刺激显着更高的抗OVA血清IgG和粪便伊加抗体相比，通过使用霍乱毒素作为阳性对照佐剂诱导。抗体反应不受胃酸预中和的影响，并持续长达七个月。共聚焦显微镜观察发现，LS可以转位到小鼠肠壁。总体而言，本研究为使用LS作为口服疫苗接种的新方法奠定了基础。
Oral vaccination can offer a painless and convenient method of vaccination. Furthermore, in addition to systemic immunity it has potential to stimulate mucosal immunity through antigen-processing by the gut-associated lymphoid tissues. In this study we propose the concept that pollen grains can be engineered for use as a simple modular system for oral vaccination. We demonstrate feasibility of this concept by using spores of Lycopodium clavatum (clubmoss) (LSs). We show that LSs can be chemically cleaned to remove native proteins to create intact clean hollow LS shells. Empty pollen shells were successfully filled with molecules of different sizes demonstrating their potential to be broadly applicable as a vaccination system. Using ovalbumin (OVA) as a model antigen, LSs formulated with OVA were orally fed to mice. LSs stimulated significantly higher anti-OVA serum IgG and fecal IgA antibodies compared to those induced by use of cholera toxin as a positive-control adjuvant. The antibody response was not affected by pre-neutralization of the stomach acid, and persisted for up to seven months. Confocal microscopy revealed that LSs can translocate in to mouse intestinal wall. Overall, this study lays the foundation of using LSs as a novel approach for oral vaccination.