Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection
Fluorescence assay based on the thioflavin T-induced conformation switch of G-quadruplexes for TET1 detection
复制标题
基于硫黄素 T 诱导的 G-四链体构象转换的荧光测定用于 TET1 检测
作者：
Chen Xue;Cheng Ying;Wang Yafen;Tang Jing;Wang Fang;Chen Zilin
10-11易位（TET）双加氧酶在胞嘧啶去甲基化以及细胞分化和转化的控制中具有重要意义。本文开发了一种荧光方法，用于高灵敏度检测 TET1（TET 双加氧酶家族的成员）。基于 ThT 诱导的 G-四链体结构的特定构象，荧光信号随着 TET1 浓度的增加而线性下降。该方法显示了筛选 TET 蛋白抑制剂以发现抗癌药物的潜在临床应用。
Ten-eleven translocation (TET) dioxygenase is of great significance in cytosine demethylation and in the control of cell differentiation and transformation. Herein, a fluorescence method has been developed for the highly sensitive detection of TET1, a member of the TET dioxygenase family. Based on the ThT-induced specific conformation of the G-quadruplex structure, the fluorescence signal decreased linearly with increasing TET1 concentration. The method shows potential clinical applications in the screening of TET protein inhibitors for anticancer drug discovery.