Adipocyte induction of preadipocyte differentiation in a gradient chamber.
Adipocyte induction of preadipocyte differentiation in a gradient chamber.
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DOI：
10.1089/ten.tec.2012.0168
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发表时间：
2012-09
期刊：
影响因子：
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通讯作者：
Ning Lai;J. K. Sims;N. Jeon;Kyongbum Lee
中科院分区：
文献类型：
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作者：
Ning Lai;J. K. Sims;N. Jeon;Kyongbum Lee
脂肪组织扩张涉及成熟脂肪细胞的扩大和通过局部驻留的前脂肪细胞的分化形成新的脂肪细胞。肥大脂肪细胞释放的因子是诱导前脂肪细胞分化的潜在因素。目前，有有限的选择，以调查这些线索孤立的混杂系统的影响。设计了一种基于梯度生成微流体通道的细胞培养系统，以实现溶液图案化，同时支持前脂肪细胞的长期培养和分化。通过选择性染色一部分均匀接种的前脂肪细胞来确认溶液图案化。使用成脂混合物梯度诱导一部分均匀接种的前脂肪细胞的分化，并建立脂肪细胞和前脂肪细胞的空间限定的共培养物。改变成脂鸡尾酒梯度产生具有不同组成的前脂肪细胞和脂肪细胞的共培养物。瞬时应用鸡尾酒梯度，然后进行基础培养基处理，显示了分化的双相诱导。分化的两个阶段与脂肪细胞大小的空间梯度相关。我们的研究结果提供了体外数据支持的大小依赖性释放的前脂肪细胞分化因子扩大的脂肪细胞。Proximal，在这项研究中开发的共培养系统可以促进控制，但在脂肪组织发育过程中脂肪细胞和前脂肪细胞之间的旁分泌相互作用的生理意义的研究。
Adipose tissue expansion involves enlargement of mature adipocytes and the formation of new adipocytes through the differentiation of locally resident preadipocytes. Factors released by the enlarged adipocytes are potential cues that induce the differentiation of the preadipocytes. Currently, there are limited options to investigate these cues in isolation from confounding systemic influences. A gradient generating microfluidic channel-based cell culture system was designed to enable solution patterning, while supporting long-term culture and differentiation of preadipocytes. Solution patterning was confirmed by selectively staining a fraction of uniformly seeded preadipocytes. An adipogenic cocktail gradient was used to induce the differentiation of a fraction of uniformly seeded preadipocytes and establish a spatially defined coculture of adipocytes and preadipocytes. Varying the adipogenic cocktail gradient generated cocultures of preadipocytes and adipocytes with different compositions. Transient application of the cocktail gradient, followed by basal medium treatment showed a biphasic induction of differentiation. The two phases of differentiation correlated with a spatial gradient in adipocyte size. Our results provide in vitro data supporting the size-dependent release of preadipocyte differentiation factors by enlarged adipocytes. Prospectively, the coculture system developed in this study could facilitate controlled, yet physiologically meaningful studies on paracrine interactions between adipocytes and preadipocytes during adipose tissue development.