Measurement of Inflammatory Chemokines in Micro-dissected Tissue Biopsy Samples by Chip-Based Immunoaffinity Capillary Electrophoresis.
Measurement of Inflammatory Chemokines in Micro-dissected Tissue Biopsy Samples by Chip-Based Immunoaffinity Capillary Electrophoresis.
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通过基于芯片的免疫亲和毛细管电泳测量显微解剖组织活检样品中的炎症趋化因子。
DOI：
10.1007/978-1-4939-4014-1_10
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Kalish,Heather
中科院分区：
文献类型：
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作者：
Phillips,TerryM;Wellner,Edward;McMohan,Shane;Kalish,Heather
为了帮助人体皮肤活检的生化分析，开发了一种基于芯片的免疫亲和毛细管电泳（ICE）系统，用于测量活检显微解剖区域的炎症趋化因子。在分离感兴趣的区域后，将组织溶解，并通过芯片内的免疫亲和盘分离感兴趣的分析物。捕获的分析物用635 nm发光激光染料原位标记，电洗脱到芯片分离通道中。所有分析物的电泳分离在2.5 min内完成，每个峰的定量通过在线LIF检测和每个峰面积的积分进行。基于芯片的系统所达到的准确度和精密度可与传统的免疫测定相媲美，并且该系统足够健壮，可以应用于临床样品的分析。此外，随着商业上抗体种类的增加，这种基于芯片的系统可以应用于各种不同的生物医学和临床分析。
To aid in the biochemical analysis of human skin biopsies, a chip-based immunoaffinity capillary electrophoresis (ICE) system has been developed for measuring inflammatory chemokines in micro-dissected areas of the biopsy. Following isolation of the areas of interest, the tissue was solubilized and the analytes of interest were isolated by the immunoaffinity disk within the chip. The captured analytes were labeled in situ with a 635 nm light-emitting laser dye and electro-eluted into the chip separation channel. Electrophoretic separation of all of the analytes was achieved in 2.5 min with quantification of each peak being performed by online LIF detection and integration of each peak area. The degree of accuracy and precision achieved by the chip-based system is comparable to conventional immunoassays and the system is robust enough to be applied to the analysis of clinical samples. Further, with the expanding array of antibodies that are commercially available, this chip-based system can be applied to a wide variety of different biomedical and clinical analyses.