Transnitrosylation Mediated by the Non-canonical Catalase ROG1 Regulates Nitric Oxide Signaling in Plants
Transnitrosylation Mediated by the Non-canonical Catalase ROG1 Regulates Nitric Oxide Signaling in Plants
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DOI：
10.1016/j.devcel.2020.03.020
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发表时间：
2020-05-18
影响因子：
11.8
通讯作者：
Zuo, Jianru
中科院分区：
文献类型：
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作者：
Chen, Lichao;Wu, Rong;Zuo, Jianru
基于氧化还原的蛋白质S-亚硝基化是调节一氧化氮（NO）信号传导的保守机制，并且主要被认为是非酶促反应。S-亚硝基化受细胞内NO水平的调节，而细胞内NO水平受S-亚硝基谷胱甘肽还原酶（GSNOR）的严格控制。然而，调节S-亚硝基化选择性的分子机制仍然难以捉摸。在这里，我们的特点是拟南芥的“阻遏物”gsnor 1（rog 1）突变，特异性抑制gsnor 1突变表型。ROG 1与非经典过氧化氢酶CAT 3相同，是一种特异性修饰GSNOR 1 Cys-10的转亚硝基酶。ROG 1的转亚硝基酶活性受一个独特的高度保守的Cys-343残基调节。一个ROG 1（C343 T）突变体显示增加过氧化氢酶，但降低转亚硝基酶活性。与这些结果一致，rog 1突变妥协的反应，在正常和压力条件下NO。我们建议ROG 1功能作为一个转亚硝基酶，以调节NO为基础的氧化还原信号在植物中。
The redox-based protein S-nitrosylation is a conserved mechanism modulating nitric oxide (NO) signaling and has been considered mainly as a non-enzymatic reaction. S-nitrosylation is regulated by the intracellular NO level that is tightly controlled by S-nitrosoglutathione reductase (GSNOR). However, the molecular mechanisms regulating S-nitrosylation selectivity remain elusive. Here, we characterize an Arabidopsis "repressor of" gsnor1 (rog1) mutation that specifically suppresses the gsnor1 mutant phenotype. ROG1, identical to the non-canonical catalase, CAT3, is a transnitrosylase that specifically modifies GSNOR1 at Cys-10. The transnitrosylase activity of ROG1 is regulated by a unique and highly conserved Cys-343 residue. A ROG1(C343T)mutant displays increased catalase but decreased transnitrosylase activities. Consistent with these results, the rog1 mutation compromises responses to NO under both normal and stress conditions. We propose that ROG1 functions as a transnitrosylase to regulate the NO-based redox signaling in plants.