Identification of the Marek's disease virus serotype 2 genes homologous to the glycoprotein B (UL27), ICP18.5 (UL28) and major DNA-binding protein (UL29) genes of herpes simplex virus type 1
Identification of the Marek's disease virus serotype 2 genes homologous to the glycoprotein B (UL27), ICP18.5 (UL28) and major DNA-binding protein (UL29) genes of herpes simplex virus type 1
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DOI：
10.1292/jvms.61.1161
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发表时间：
1999-10-01
影响因子：
1.2
通讯作者：
Mikami, T
中科院分区：
文献类型：
--
作者：
Kato, K;Jang, HK;Mikami, T
我们测定了非致病性马立克氏病病毒2型(MDV2)株HPRS24糖蛋白B(GB)(UL27)、ICP18.5(UL28)和主要DNA结合蛋白(MDBP)(UL29)基因与单纯疱疹病毒1型(HSV-1)的同源性。序列分析表明，MDV2、ICP18.5和MDBP在蛋白质中的重要动机是保守的，而在MDV2基因组中没有发现存在于其他甲型疱疹病毒UL29和UL30基因之间的病毒DNA复制起始序列。Northern印迹分析还表明，在MDV2感染的细胞中，8.9、5.0和2.6kb的转录本实际上是从测序区转录出来的。MDV2 UL28和UL29基因在其他血清型MDV中未见报道。
We determined the nucleotide sequence of non-pathogenic Marek's disease virus serotype 2 (MDV2) strain HPRS24 glycoprotein B (gB) (UL27), ICP18.5 (UL28) and major DNA-binding protein (MDBP) (UL29) genes homologous to herpes simplex virus type 1 (HSV-1). The sequence data revealed that important motives in the proteins are conserved in MDV2 ICP18.5 and MDBP, however the sequence of viral DNA replication origin which exists in the regions between the UL29 and UL30 genes of other alphaherpesviruses was not found in the regions of the MDV2 genome. By northern blot analyses, we also demonstrated that 8.9, 5.0 and 2.6 kb transcripts were actually transcribed from the sequenced region in MDV2-infected cells. The MDV2 UL28 and UL29 genes have not been reported in other serotypes of MDV.