HEPATOCYTE PROLIFERATION AS AN INDICATOR OF OUTCOME IN ACUTE ALCOHOLIC HEPATITIS
HEPATOCYTE PROLIFERATION AS AN INDICATOR OF OUTCOME IN ACUTE ALCOHOLIC HEPATITIS
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DOI：
10.1016/s0140-6736(94)92025-7
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发表时间：
1994-04-02
期刊：
影响因子：
168.9
通讯作者：
LAU, JYN
中科院分区：
文献类型：
--
作者：
FANG, JWS;BIRD, GLA;LAU, JYN
为了确定急性酒精性肝炎患者的临床结果是否与肝脏的再生能力相关，我们研究了 25 名前瞻性招募的急性酒精性肝炎患者的肝活检标本，研究了两种增殖标志物（增殖细胞核抗原和人组蛋白）的 mRNA 表达，以及调节肝细胞增殖的转化生长因子 α（TGFα）和 TGFβ1 以及肝细胞生长因子（HGF）的 mRNA 表达。 19 名患者 (76%) 的肝细胞中存在 0-80%，偶尔存在于肝窦细胞和胆管上皮中。存活 6 个月的患者比未存活的患者具有更高的增殖标志物表达 (p < 0.01)。 TGFα 在肝细胞和胆管上皮中检测到，而 TGFβ1 主要在肝窦细胞中检测到，并且与静脉周围纤维化相关。存活 6 个月的患者的 TGF 表达高于未存活者 (p < 0.02)。在 7 名患者的肝窦细胞中检测到 HGF，并与生存相关 (p < 0.01)。这些数据表明，肝细胞增殖可能与肝营养因子表达模式有关，是急性酒精性肝炎预后的良好指标。
To determine whether clinical outcome in patients with acute alcoholic hepatitis is related to the regenerative capability of the liver, liver biopsy specimens from 25 prospectively recruited patients with acute alcoholic hepatitis were studied for hepatic expression of mRNA for two proliferation markers, proliferating cell nuclear antigen and human histone, and for transforming growth factor alpha (TGFalpha) and TGFbeta1 and hepatocyte growth factor (HGF), which regulate hepatocyte proliferation.Proliferation markers were detected to varying degrees in 0-80% of hepatocytes and occasionally in sinusoidal cells and bile-duct epithelium in 19 patients (76%). Patients who survived for 6 months had greater expression of proliferation markers than those who did not survive (p < 0.01). TGFalpha was detected in hepatocytes and bile-duct epithelium, whereas TGFbeta1 was detected mainly in sinusoidal cells and was associated with perivenular fibrosis. Patients who survived for 6 months had greater expression of TGFs than non-survivors (p < 0.02). HGF was detected in sinusoidal cells in 7 patients and correlated with survival (p < 0.01).These data indicate that hepatocyte proliferation, which is possibly related to the pattern of hepatotrophic factor expression, is a good indicator of outcome in acute alcoholic hepatitis.