In vitro anti-proliferative effect and in vivo antitumor action of daphnetin in different tumor cells
In vitro anti-proliferative effect and in vivo antitumor action of daphnetin in different tumor cells
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DOI：
10.24875/ciru.20000197
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发表时间：
2020-12-01
期刊：
影响因子：
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通讯作者：
Tóvarí, József
中科院分区：
文献类型：
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作者：
Jiménez-Orozco, F. Alejandro;Ranđelović, Ivan;Tóvarí, József
背景：瑞香素（7,8-二氢香豆素）的抗炎作用已得到充分证明，但瑞香素作为抗癌药物的潜力存在争议，且尚未得到充分探索。材料和方法：在这项工作中，我们通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定评估了瑞香素在三种细胞系中的体外抗增殖作用，以及其在四种不同类型的小鼠肿瘤中的体内抗肿瘤作用。结果：根据体外和体内结果之间的相关性，测试的细胞类型对化合物具有不同的敏感性。根据瑞香素的体外抗增殖效力排列如下：B16黑色素瘤细胞（抑制浓度50[IC50]=54+/-2.8μM）>米托蒽醌（MXT）乳腺癌细胞（IC50=74+/-6.4μM）>C26结肠癌细胞（IC50=108+/-7.3μM） 米）。在体内，瑞香素的最佳抗肿瘤剂量为40 mg/kg，抑制程度如下：B16肿瘤（48%）>MXT肿瘤（40%）>S180纤维肉瘤肿瘤（30%）>C26肿瘤（20%）。结论：我们的结果表明瑞香素可能作为辅助药物提高常规化疗的疗效。
Background: The anti-inflammatory effects of daphnetin (7,8-dihidroxicoumarin) have been well-documented, but the potential of daphnetin as an anticancer agent is controversial and remains insufficiently explored. Material and methods: In this work, we evaluated the in vitro anti-proliferative effect of daphnetin in three cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, as well as its in vivo antitumor effect in four different types of mouse tumor. Results: With a correlation between in vitro and in vivo results, the tested cell types have different sensitivity to the compound. The following cell lines are arranged according to the in vitro anti-proliferative potency of daphnetin: B16 melanoma cells (inhibitory concentrations 50 [IC50]= 54 +/- 2.8 mu M) > mitoxantrone (MXT) breast adenocarcinoma cells (IC50 = 74 +/- 6.4 mu M) > C26 colon carcinoma cells (IC50 = 108 +/- 7.3 mu M). In vivo, the optimal antitumor dose of daphnetin was 40 mg/kg and the magnitudes of inhibition were the following: B16 tumor (48%) > MXT tumor (40%) > S180 fibrosarcoma tumor (30%) > C26 tumor (20%). Conclusion: Our results indicate that daphnetin might have an impact as adjuvant to improve the effectiveness of conventional chemotherapy.