The tyrosine-containing cytoplasmic tail of CD1b is essential for its efficient presentation of bacterial lipid antigens
The tyrosine-containing cytoplasmic tail of CD1b is essential for its efficient presentation of bacterial lipid antigens
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DOI：
10.1016/s1074-7613(00)80539-7
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发表时间：
1998-03-01
期刊：
影响因子：
32.4
通讯作者：
Porcelli, SA
中科院分区：
文献类型：
--
作者：
Jackman, RM;Stenger, S;Porcelli, SA
CD1b 是一种抗原呈递分子，介导特定 T 细胞对细菌脂质和糖脂抗原的识别。我们证明 CD1b 的九个氨基酸胞质尾包含其正常内体靶向所需的所有信号，并且单个胞质酪氨酸是靶向基序的关键组成部分。缺乏内体靶向基序的 CD1b 突变形式在细胞表面高水平表达，但无法有效呈递从外源或活细胞内生物体获得的脂质抗原。这些结果定义了 CD1b 靶向基序在生理环境中的功能作用，并证明了其在将该抗原呈递分子递送至适当的细胞内区室中的重要性。
CD1b is an antigen-presenting molecule that mediates recognition of bacterial lipid and glycolipid antigens by specific T cells. We demonstrate that the nine-amino acid cytoplasmic tail of CD1b contains all of the signals required for its normal endosomal targeting, and that the single cytoplasmic tyrosine is a critical component of the targeting motif. Mutant forms of CD1b lacking the endosomal targeting motif are expressed at high levels on the cell surface but are unable to efficiently present lipid antigens acquired either exogenously or from live intracellular organisms. These results define the functional role of the CD1b targeting motif in a physiologic setting and demonstrate its importance in delivery of this antigen-presenting molecule to appropriate intracellular compartments.