Ontogenetic development of the water channel protein AQP5 in mouse salivary gland tissue
Ontogenetic development of the water channel protein AQP5 in mouse salivary gland tissue
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DOI：
10.1007/s00441-023-03762-w
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发表时间：
2023-03-15
影响因子：
3.6
通讯作者：
Nonaka，Naoko
中科院分区：
文献类型：
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作者：
Matsuyama，Kayo;Fujikawa，Kaoru;Nonaka，Naoko
水通道蛋白（AQP）是在许多组织类型的细胞膜上表达的一个通道蛋白家族。作为水通道，它们使水分子选择性渗透，从而在水通过质膜的运输中发挥重要作用。AQP有许多亚型，其中AQP5在唾液腺中表达。AQP5在动物模型胎儿发育和出生后不同唾液腺细胞中的表达和定位已经阐明了AQP5的生理功能，但在成人期的后续变化尚不清楚。众所周知，唾液分泌随着年龄的增长而减少，但AQP5的活性和功能如何随着年龄的增长而变化，包括性别差异，尚不清楚。在本研究中，我们采集了幼年（8周龄）和老年（12个月龄）小鼠的腮腺、下颌骨和舌下腺样本，研究年龄和性别相关的AQP5表达差异的影响。在所有腺体类型的细胞膜上都检测到阳性的荧光免疫染色，并且在雌雄幼鼠中都有所增强。Western blot分析显示，AQP5的表达水平在雄性和雌性动物中都随着年龄的增长而降低。相反，AQP5基因的表达水平随着年龄的增长没有明显变化，但在两性的下颌下腺细胞、老年雌性小鼠的腮腺细胞和年轻雄性小鼠的舌下腺细胞中均较高。
Aquaporins (AQP) are a family of channel proteins expressed in the cell membranes of many tissue types. As water channels, they enable the selective permeation of water molecules and thus play an important role in water transport through the plasma membrane. There are numerous AQP sub-types, among which AQP5 is expressed in the salivary glands. The expression and localization of AQP5 in different salivary gland cells of animal models during fetal development and after birth have enabled the physiological functions of AQP5 to be elucidated, but subsequent changes in the adult phase are unknown. It is known that saliva production tends to decrease with age, but it is unclear how AQP5 activity and function changes developmentally, from young to old including gender differences. In the present study, we sampled the parotid, submandibular, and sublingual glands from young (8 weeks old) and aged (12 months old) mice of both sexes to study the effects of age- and sex-related differences in AQP5 expression. Positive fluorescence immunostaining was detected in the membranes of cells from all gland types, and this was enhanced in juvenile mice from both sexes. Western blot analyses revealed that AQP5 expression levels tended to decrease with age in both male and female animals. Conversely, AQP5 gene expression levels did not change significantly with aging, but were found to be high in submandibular gland cells of both sexes, in parotid gland cells of older female mice, and in the sublingual gland cells of young male mice.