Controlling the Translocation of Single-Stranded DNA through α-Hemolysin Ion Channels Using Viscosity
Controlling the Translocation of Single-Stranded DNA through α-Hemolysin Ion Channels Using Viscosity
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DOI：
10.1021/la803556p
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发表时间：
2009-01-20
期刊：
影响因子：
3.9
通讯作者：
White, Henry S.
中科院分区：
文献类型：
--
作者：
Kawano, Ryuji;Schibel, Anna E. P.;White, Henry S.
在含有 1 M KCl 的甘油/水混合物中研究了单链 DNA 通过 α 溶血素 (α-HL) 通道的易位。使用玻璃纳米孔膜作为脂质双层支持物的实验表明，相对于水溶液，聚（脱氧腺苷酸）、聚（脱氧胞苷酸）和聚（脱氧胸苷酸）50聚体的易位速度在63/37（vol%）甘油/水混合物中降低了大约20倍。 α-HL的离子电导和多核苷酸进入蛋白质通道的速率也随着粘度的增加而降低。通过调节粘度精确控制易位参数为改进基于离子通道记录的测序方法提供了潜在的手段。
Translocation of single-stranded DNA through alpha-hemolysin (alpha-HL) channels is investigated in glycerol/water mixtures containing 1 M KCl. Experiments using glass nanopore membranes as the lipid bilayer support demonstrate that the translocation velocities of poly(deoxyadenylic acid), poly(deoxycytidylic acid), and poly(deoxythymidylic acid) 50-mers are decreased by a factor of similar to 20 in a 63/37 (vol %) glycerol/water mixture, relative to aqueous solutions. The ion conductance of alpha-HL and the entry rate of the polynucleotides into the protein channel also decrease with increasing viscosity. Precise control of translocation parameters by adjusting viscosity provides a potential means to improve sequencing methods based on ion channel recordings.