Genetic regulation of early survival and cyst number after peroral Toxoplasma gondii infection of A x B/B x A recombinant inbred and B10 congenic mice.
Genetic regulation of early survival and cyst number after peroral Toxoplasma gondii infection of A x B/B x A recombinant inbred and B10 congenic mice.
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DOI：
10.4049/jimmunol.143.9.3031
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发表时间：
1989-11
影响因子：
4.4
通讯作者：
R. McLeod;E. Skamene;Charles R. Brown;P. Eisenhauer;D. Mack
中科院分区：
文献类型：
--
作者：
R. McLeod;E. Skamene;Charles R. Brown;P. Eisenhauer;D. Mack
用抗弓形虫A/J（A）和易感弓形虫C57 BL/6 J（B）小鼠的重组近交系、A/J和C57 BL/6 J小鼠杂交的F1代以及B10背景的同种小鼠，研究了经口感染弓形虫后存活率和脑囊肿数两个性状的遗传学。对A × B/B × A重组小鼠存活率的品系分布模式分析表明，存活率受至少5个基因的调控。其中一个基因似乎与H-2复合体有关，另一个基因与一个尚未定位的控制鼠痘病毒抗性的基因有关。还分析了定义的性状与弓形虫包囊形成的抗性或易感性的关联。囊肿数量受17号染色体上H-2复合物0 - 4厘摩范围内的一个位点调节（p = 0.001）。具有H-2a单倍型的小鼠是抗性的，而具有H-2b单倍型的小鼠是易感的。该分析还表明1号染色体上的Bcg基因座可能影响孢囊数量（图距= 12厘摩，p = 0.05）。抗孢囊形成是显性性状。为了分析H-2和Bcg基因座对包囊数量的相关作用，研究了具有已知H-2和Bcg基因型的C57BL 10（B10）同源系小鼠。这些研究表明，H-2复合体位点对孢囊数量有主要影响。
Genetics of two traits, survival and brain cyst number after peroral Toxoplasma gondii infection, were studied by using recombinant inbred strains of mice derived from resistant A/J (A) and susceptible C57BL/6J (B) progenitors, F1 progeny of crosses between A/J and C57BL/6J mice, and congenic mice (B10 background). Analysis of strain distribution pattern of survival of A x B/B x A recombinant mice indicated that survival is regulated by a minimum of five genes. One of these genes appears to be linked to the H-2 complex and another is related to an as yet unmapped gene controlling resistance to Ectromelia virus. Associations of defined traits with resistance or susceptibility to Toxoplasma cyst formation were also analyzed. Cyst number is regulated by a locus on chromosome 17 within 0 to 4 centimorgans of the H-2 complex (p = 0.001). Mice with the H-2a haplotype are resistant and those with the H-2b haplotype are susceptible. This analysis also indicated that the Bcg locus on chromosome 1 may effect cyst number (map distance = 12 centimorgans, p = 0.05). Resistance to cyst formation is a dominant trait. To analyze relative roles of H-2 and Bcg loci on cyst numbers, C57BL10 (B10)-derived congenic strains of mice with known H-2 and Bcg type were studied. These studies indicated that the H-2 complex locus has the primary effect on cyst number.