Portable nanofiber meshes dictate cell orientation throughout three-dimensional hydrogels.
Portable nanofiber meshes dictate cell orientation throughout three-dimensional hydrogels.
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DOI：
10.1016/j.nano.2010.12.011
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Ying Yang;I. Wimpenny;M. Ahearne
中科院分区：
文献类型：
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作者：
Ying Yang;I. Wimpenny;M. Ahearne
在这项研究中，一种新的技术，控制个别细胞的取向，使用三维（3D）水凝胶内的网格。将高度对齐且易碎的电纺纳米纤维（平均直径500 nm）制造成便携式且可折叠的网，其平均线密度为每100 μm 45根纳米纤维，厚度范围为0.5 μ m至3.0 μm。通过一个简单的和可重复的制造过程，可通过自下而上，逐层组装过程将三维网格纳入3D水凝胶中，从而形成宏观和高度组织化的支架。纳米纤维以非常精确的方式决定了单个细胞的细胞骨架的取向，允许在整个水凝胶厚度上改变细胞群的取向。添加纳米纤维影响细胞表型和蛋白质合成。这种纳米纤维-细胞-水凝胶复合材料能够复制许多天然组织中发现的细胞和基质结构，为再生医学和生物工程中的电纺纳米纤维提供了一种新的方案。来自临床编辑：一种新的协议，高度组织化的三维水凝胶中的网状结构，可用于指导覆盖细胞群的细胞骨架方向，表型和蛋白质合成。纳米纺基质为受控组织生物工程和再生医学应用提供了重大进展。
In this study, a new technique that controls individual cell orientation using nanofiber meshes within three-dimensional (3D) hydrogels is reported. Highly aligned and fragile electrospun nanofibers (average diameter 500 nm) were manufactured into portable and handleable meshes with average line density of 45 nanofibers per 100 μm and thickness ranging between 0.5 and 3.0 μm. Through a facile and reproducible fabrication process, the nanofiber meshes can be incorporated into 3D hydrogels via a bottom-up, layer-by-layer assembly process, resulting in macroscopic and highly organized scaffolds. The nanofibers dictated the orientation of the cytoskeleton of individual cells in a very precise manner, allowing altering of the orientation of a cell population throughout the thickness of the hydrogel. Addition of nanofibers affected cell phenotype and protein synthesis. This nanofiber-cell-hydrogel composite enables replication of the cellular and matrix architecture found in many natural tissues, offering a novel protocol for electrospun nanofibers in regenerative medicine and bioengineering. FROM THE CLINICAL EDITOR: A novel protocol for highly organized nanofiber meshes incorporated into 3D hydrogels can be used to direct the overlying cell population cytoskeleton direction, phenotype, and protein synthesis. Nanospun matrices offers a significant advancement for controlled tissue bioengineering and regenerative medicine applications.