Metagenomic assembly provides a deep insight into the antibiotic resistome alteration induced by drinking water chlorination and its correlations with bacterial host changes
Metagenomic assembly provides a deep insight into the antibiotic resistome alteration induced by drinking water chlorination and its correlations with bacterial host changes
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DOI：
10.1016/j.jhazmat.2019.120841
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发表时间：
2019-11-05
影响因子：
13.6
通讯作者：
Zhang, Xu-Xiang
中科院分区：
文献类型：
--
作者：
Jia, Shuyu;Wu, Jialu;Zhang, Xu-Xiang
氯化可以促进饮用水中特定抗生素抗性基因（ARG）的富集，但潜在的分子生态机制仍然未知，这可能会阻碍对由此产生的健康风险的评估和控制。在本研究中，宏基因组组装和 Resfams 注释用于分析全面饮用水处理和运输系统中 ARG、移动遗传元件 (MGE) 及其细菌宿主的共现模式，以及潜在病原体与抗生素抗性组的相关性。饮用水中有七种涉及不同抗性机制的 ARG 类型，氯化提高了 ARG 的总丰度 (p < 0.05)。编码抗性结节细胞分裂和 ATP 结合盒抗生素外排泵的 ARG 在所有样品中占主导地位，并且主要负责 ARG 的积累。氯化后，ARG 主要由主要的鞘氨醇单胞菌属、极化单胞菌属、丝菌属、食酸菌属、假单胞菌属和 Fluviicola 携带。此外，细菌宿主和 MGE 的富集极大地促进了抗生素抗性组的改变。产碱假单胞菌携带多种 ARG，被鉴定为氯化饮用水中的潜在病原体。这些发现为宿主-ARG 关系以及饮用水氯化过程中抗性组改变的机制提供了新的见解。
Chlorination can contribute to the enrichment of specific antibiotic resistance genes (ARGs) in drinking water, but the underlying molecular ecological mechanisms remain unknown, which may hinder the assessment and control of the resulting health risks. In this study, metagenomic assembly and Resfams annotation were used to profile the co-occurrence patterns of ARGs, mobile genetic elements (MGEs) and their bacterial hosts, as well as the correlations of potential pathogens with the antibiotic resistome, in a full-scale drinking water treatment and transportation system. Seven ARG types involved in different resistance mechanisms occurred in drinking water and chlorination enhanced the total abundance of the ARGs (p < 0.05). The ARGs encoding resistance-nodulation-cell division and ATP-binding cassette antibiotic efflux pumps predominated in all the samples and were primarily responsible for the ARG accumulation. After chlorination, the ARGs were primarily carried by predominant Sphingomonas, Polaromonas, Hyphomicrobium, Acidovorax, Pseudomonas and Fluviicola. Further, enrichment of the bacterial hosts and MGEs greatly contributed to alteration of the antibiotic resistome. Pseudomonas alcaligenes, carrying multiple ARGs, was identified as a potential pathogen in the chlorinated drinking water. These findings provide novel insights into the host-ARG relationship and the mechanism underlying the resistome alteration during drinking water chlorination.