Newly designed hemostatic technology based on photocurable gelatin.
Newly designed hemostatic technology based on photocurable gelatin.
复制标题
新设计的基于光固化明胶的止血技术。
DOI：
--
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
T. Matsuda
中科院分区：
文献类型：
--
作者：
Y. Nakayama;T. Matsuda
在这篇文章中，作者提出了一种新的光化学驱动的止血技术，使用光固化明胶部分衍生的光反应性咕吨染料（荧光素，曙红，玫瑰红）和亲水性双功能大分子单体。所开发的止血胶由染料衍生明胶（20重量%）、聚（乙二醇）二丙烯酸酯（10重量%）和抗坏血酸（0.3重量%）组成，所有这些均溶解在盐水溶液中。通过可见光照射止血胶，由于染料敏化光交联和光接枝聚合，在十分之几秒内产生溶胀凝胶。辐照时间的增加导致凝胶产率增加和水溶胀性降低。将在剖腹手术中损伤的大鼠肝脏涂上止血胶。在通过光纤进行可见光照射时，涂覆的粘性溶液立即转化为溶胀的凝胶，同时完成止血。组织学检查显示，术后7天，损伤区域几乎没有明胶残留，出现瘢痕，几乎没有坏死，周围组织中有炎性细胞浸润，组织再生进行良好。在腹腔镜手术期间，使用专门设计的纤维镜证明止血胶在肝脏表面的原位凝胶化。
In this article, the authors present a novel photochemically driven hemostatic technology using photocurable gelatins partially derivatized with photoreactive xanthene dyes (fluorescein, eosin, and rose bengal) and a hydrophilic difunctional macromer. The developed hemostatic glue consisted of dye derivatized gelatin (20 wt%), poly(ethylene glycol) diacrylate (10 wt%), and ascorbid acid (0.3 wt%), all of which were dissolved in a saline solution. Irradiation of the hemostatic glue by visible light produced a swollen gel within a few tenths of a second due to dye sensitized photo-crosslinking and photograft polymerization. An increase in irradiation time resulted in an increased gel yield and reduced water swellability. A rat liver injured on laparotomy was coated with the hemostatic glue. Upon visible light irradiation through an optical fiber, the coated viscous solution was immediately converted to a swollen gel and, concomitantly, hemostasis was completed. Histologic examination showed that, at 7 days after surgery, little gelatin remained in the injured region, scarring with little necrosis occurred, and inflammatory cells infiltrated from the surrounding tissue and tissue regeneration proceeded well. During laparoscopic surgery, in situ gelation of the hemostatic glue on the liver surface was demonstrated using a specially designed fiberscope.