Sperm and seminal plasma proteomics of high- versus low-fertility Holstein bulls
Sperm and seminal plasma proteomics of high- versus low-fertility Holstein bulls
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DOI：
10.1016/j.theriogenology.2018.11.032
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发表时间：
2019-03-01
期刊：
影响因子：
2.8
通讯作者：
Kastelic, J. P.
中科院分区：
文献类型：
--
作者：
Kasimanickam, R. K.;Kasimanickam, V. R.;Kastelic, J. P.
精子是高度专业化的区室细胞，具有独特的组成、形态和功能特性，包括经历动态蛋白质重塑和表面修饰的质膜。精浆是一种高度复杂的生物液体，含有蛋白质、氨基酸、酶、果糖和其他碳水化合物、脂质、主要矿物质和微量元素。精浆蛋白参与精浆渗透压和 pH 值的调节、离子、脂质和激素的运输。目的是比较具有不同生育能力的公牛的精子和精浆蛋白质组，并将差异与生物过程联系起来。精液采集自高生育力或低生育力的公牛（每个类别 4 头公牛）。分离、纯化精子和精浆蛋白，进行二维凝胶电泳、蛋白鉴定和本体论。在精子和精浆中，高生育力公牛的精子蛋白结合剂（BSP）-1、-3和-5、spermadhesin-1、ALB、TIMP、AKI和PEBP1比低生育力公牛更高（P < 0.05），而蛋白质CLU、Ca5和8、ELSPbP1和PSMA6在低生育力公牛的精子和精浆中比高生育力公牛更丰富（P < 0.05）。 0.05）。此外，与低生育力公牛相比，高生育力公牛精子中的 HSP90、ZFP34、IFNRF4、BCL62、NADHD、TUBB3 和组蛋白 H1 丰度更高。高繁殖力和低繁殖力公牛差异表达的蛋白质的两个关键生物过程是代谢过程和生物调节。不同蛋白质最突出的分子功能是结合、催化和受体活性。不同蛋白质的主要细胞成分是细胞、细胞外和质膜。由于高生育力公牛与低生育力公牛的蛋白质含量不同，我们推断高生育力和低生育力精液之间生育所需的相关精子功能的效率也可能不同。总之，高生育力公牛和低生育力公牛之间在精子和精浆蛋白丰度方面的差异可能导致了生育力的差异。 (C) 2018 Elsevier Inc. 保留所有权利。
Sperm are highly specialized compartmentalized cells, with unique compositional, morphological and functional properties, including a plasma membrane that undergoes dynamic protein remodeling and surface modifications. Seminal plasma is a highly complex biological fluid containing proteins, amino acids, enzymes, fructose and other carbohydrates, lipids, major minerals and trace elements. Seminal plasma proteins are involved in regulation of osmotic pressure and pH of seminal plasma, transport of ions, lipid and hormones. The objective was to compare sperm and seminal plasma proteomes of bulls with differing fertility and to relate differences to biological processes. Semen was collected from bulls with high or low fertility (4 bulls in each category). Sperm and seminal plasma proteins were isolated, purified, subjected to 2-D gel electrophoresis, protein identification and ontology. In sperm and seminal plasma, binder of sperm proteins (BSP)-1, -3 and -5, and spermadhesin-1, ALB, TIMP, AKI and PEBP1 were higher for high-versus low-fertility bulls (P < 0.05), whereas proteins CLU, Ca5 and 8, ELSPbP1, and PSMA6 were more abundant in sperm and seminal plasma of low-versus high-fertility bulls (P < 0.05). Further, HSP90, ZFP34, IFNRF4, BCL62, NADHD, TUBB3 and Histone H1 were in greater abundance in sperm of high-compared with low-fertility bulls. The two key biological processes of proteins differentially expressed in high-and low-fertility bulls were metabolic processes and biological regulation. The most prominent molecular functions for proteins that differed are binding, catalytic and receptor activities. The main cellular components for proteins that differed are cellular, extracellular, and plasma membrane. Since protein content differed in high-versus low-fertility bulls, we inferred that the efficiency of associated sperm functions that are necessary for fertility may also differ between high-and low-fertility semen. In conclusion, differences between high-and low-fertility bulls regarding abundance of sperm and seminal plasma proteins likely contributed to differences in fertility. (C) 2018 Elsevier Inc. All rights reserved.