Synergistic effects of high dietary calcium and exogenous parathyroid hormone in promoting osteoblastic bone formation in mice.
Synergistic effects of high dietary calcium and exogenous parathyroid hormone in promoting osteoblastic bone formation in mice.
复制标题
高膳食钙与外源性甲状旁腺激素促进小鼠成骨细胞骨形成的协同作用
DOI：
10.1017/s0007114514004309
复制
发表时间：
2015-03-28
影响因子：
3.6
通讯作者：
Ren, Yongxin
中科院分区：
文献类型：
--
作者：
Feng, Yuxu;Zhou, Min;Zhang, Qunhu;Liu, Huan;Xu, Yong;Shu, Lei;Zhang, Jue;Miao, Dengshun;Ren, Yongxin
本研究探讨了高钙饮食和外源性甲状旁腺激素1-34片段（PTH 1-34）对成年小鼠骨形成的协同作用及其机制。给成年雄性小鼠喂食正常饮食、高钙饮食、PTH处理的饮食或高钙饮食结合皮下注射PTH 1-34（80 μg/kg/d）4周。骨密度，骨小梁体积，成骨细胞数量，碱性磷酸酶（ALP）和I型胶原阳性面积，成骨细胞骨形成相关基因和蛋白质的表达水平显着增加，高钙饮食，PTH治疗的饮食，甚至更显着，高钙饮食结合PTH。破骨细胞数量、破骨细胞表面积、破骨细胞核因子-κB配体受体激活因子（RANKL）与骨保护素（OPG）的比值在高钙饮食治疗组降低，在PTH治疗组升高，但在联合治疗组无变化。此外，第三代成骨细胞用高Ca（5 mm）、PTH 1-34（10 - 8 m）或高Ca与PTH 1-34组合处理。成骨细胞的活力和ALP活性增加，无论是高钙处理或PTH处理的文化，甚至更显着，在高钙加PTH处理的文化，与成骨细胞增殖和分化相关的基因和蛋白质的表达水平的一致上调。这些结果表明，膳食钙和PTH通过刺激成骨细胞增殖和分化，在促进成骨细胞骨形成中发挥协同作用。
In the present study, we investigated whether high dietary Ca and exogenous parathyroid hormone 1–34 fragments (PTH 1–34) have synergistic effects on bone formation in adult mice, and explored the related mechanisms. Adult male mice were fed a normal diet, a high-Ca diet, a PTH-treated diet, or a high-Ca diet combined with subcutaneously injected PTH 1–34 (80 μg/kg per d) for 4 weeks. Bone mineral density, trabecular bone volume, osteoblast number, alkaline phosphatase (ALP)- and type I collagen-positive areas, and the expression levels of osteoblastic bone formation-related genes and proteins were increased significantly in mice fed the high-Ca diet, the PTH-treated diet, and, even more dramatically, the high-Ca diet combined with PTH. Osteoclast number and surface and the ratio of receptor activator for nuclear factor-κB ligand (RANKL):osteoprotegerin (OPG) were decreased in the high-Ca diet treatment group, increased in the PTH treatment group, but not in the combined treatment group. Furthermore, third-passage osteoblasts were treated with high Ca (5 mm), PTH 1–34 (10− 8 m) or high Ca combined with PTH 1–34. Osteoblast viability and ALP activity were increased in either the high Ca-treated or PTH-treated cultures and, even more dramatically, in the cultures treated with high Ca plus PTH, with consistent up-regulation of the expression levels of osteoblast proliferation and differentiation-related genes and proteins. These results indicate that dietary Ca and PTH play synergistic roles in promoting osteoblastic bone formation by stimulating osteoblast proliferation and differentiation.