THE EFFECT OF DIFFERENT STRONTIUM CONCENTRATIONS ON THE EFFICACY OF CHLORHEXIDINE FLUORIDE STRONTIUM GEL IN PREVENTING ENAMEL SOFTENING INVITRO
THE EFFECT OF DIFFERENT STRONTIUM CONCENTRATIONS ON THE EFFICACY OF CHLORHEXIDINE FLUORIDE STRONTIUM GEL IN PREVENTING ENAMEL SOFTENING INVITRO
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DOI：
10.1016/0003-9969(93)90194-q
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发表时间：
1993-02-01
影响因子：
3
通讯作者：
RAISANEN, J
中科院分区：
文献类型：
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作者：
SPETSHAPPONEN, S;LUOMA, H;RAISANEN, J
比较了氯己定（0.20%）-氟化物（0.16%）凝胶中锶浓度为0、15、50和250份/10（6）时防止细菌发酵过程中牛牙釉质软化的效果。将新鲜远缘链球菌细胞悬浮在含有3.3%蔗糖的缓冲溶液（pH6.0）中，并在覆盖有10穆尔的釉质板的顶部上离心形成噬斑样层。孵育24 h后，所有试验组中液体的pH值下降均显著小于安慰剂组。锶似乎对pH下降有抑制作用。所有测试凝胶显著防止细菌发酵期间釉质的软化和溶解，这与锶浓度直接相关。培养后细胞和液体中的钙和磷的量在含250份/10（6）锶的组中最小。在没有细菌的第二个实验中，用10穆尔含有氯己定和NaF的凝胶覆盖新的釉质板，其中含有0和250份/10（6）锶，以研究锶的保护作用。凝胶处理24小时后，将每个釉质板溶解在1 ml 0.1M乳酸（pH5.0）中2小时。与对照组和安慰剂组相比，两个试验组中从釉质中溶解的钙和磷显著更少。然而，安慰剂凝胶也显著防止钙和磷溶解。这些结果表明，在氯己定-氟化物凝胶中加入锶可提高该组合预防龋齿样牙釉质软化的能力。含锶凝胶对细菌产酸的抑制可以解释这种增强的釉质保护。
The effects were compared of strontium concentrations of 0, 15, 50 and 250 parts/10(6) in chlorhexidine(0.20%)-fluoride(0.16%) gel in preventing the softening of bovine enamel during bacterial fermentation. Fresh Streptococcus sobrinus cells were suspended in a buffer solution (pH 6.0) with 3.3 % sucrose and centrifuged as a plaque-like layer on the top of enamel slabs covered with 10 mul gel. After 24 h incubation, the pH fall of the fluid was significantly smaller in all test groups than in the placebo group. Strontium seemed to have an inhibitory effect on the pH fall. All test gels significantly prevented softening and dissolution of enamel during bacterial fermentation, which was directly related to strontium concentration. The amounts of calcium and phosphorus in the cells and fluid after incubation were the smallest in the group with 250 parts/10(6) strontium. In a second experiment without bacteria, new enamel slabs were covered with 10 mul gel containing chlorhexidine and NaF with 0 and 250 parts/10(6) strontium to study the protective effect of strontium. After 24 h gel treatment, each enamel slab was dissolved in 1 ml 0.1 M lactic acid, pH 5.0, for 2 h. Significantly less calcium and phosphorus were dissolved from the enamel in both test groups than in the control and placebo groups. However, the placebo gel also prevented calcium and phosphorus dissolution significantly. These results suggest that the addition of strontium to chlorhexidine-fluoride gel improves the ability of this combination to prevent caries-like enamel softening. The inhibition of bacterial acid production by the strontium-containing gel may explain this enhanced protection of enamel.