ChIP-seq identifies McSLC35E2 as a novel target gene of McNrf2 in Mytilus coruscus, highlighting its role in the regulation of oxidative stress response in marine mollusks.
ChIP-seq identifies McSLC35E2 as a novel target gene of McNrf2 in Mytilus coruscus, highlighting its role in the regulation of oxidative stress response in marine mollusks.
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DOI：
10.3389/fphys.2023.1282900
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发表时间：
2023
影响因子：
4
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中科院分区：
文献类型：
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NF-E2 相关因子 2 (Nrf2) 在氧化调节过程中发挥着至关重要的作用，它可以触发数百种抗氧化元素来对抗异生素。在之前的研究中，我们从海洋贻贝Mytilus coruscus中鉴定出Nrf2，结果表明McNrf2有效保护贻贝免受苯并芘（Bap）诱导的氧化应激。为了更深入地探究其潜在机制，我们利用染色质免疫沉淀测序（ChIP-seq）技术系统地鉴定了 McNrf2 的潜在新靶基因。总共筛选了 3,465 个潜在靶基因，其中 219 个拥有位于启动子区域的结合位点。在后续的实验验证中，通过双荧光素酶和qRT-PCR检测证实，McNrf2的候选靶基因McSLC35E2表现出McNrf2的负调控作用。此外，酶活性测试表明McNrf2可以通过抑制McSLC35E2来抵消Bap诱导的氧化应激。目前的研究为 ChIP-seq 技术在海洋软体动物研究中的应用提供了宝贵的见解，加深了我们对 Nrf2 在抗氧化防御机制中关键作用的理解，并强调了 SLC35E2 在海洋无脊椎动物氧化应激反应的高度复杂调节中的重要性。
NF-E2-related factor 2 (Nrf2) plays a crucial role in the oxidative regulatory process, which could trigger hundreds of antioxidant elements to confront xenobiotics. In the previous study, we identified Nrf2 from the marine mussel Mytilus coruscus, and the findings demonstrated that McNrf2 effectively protected the mussels against oxidative stress induced by benzopyrene (Bap). In order to delve deeper into the underlying mechanism, we utilized Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) technology to systematically identify potential novel target genes of McNrf2. A total of 3,465 potential target genes were screened, of which 219 owned binding sites located within the promoter region. During subsequent experimental verification, it was found that McSLC35E2, a candidate target gene of McNrf2, exhibited negative regulation by McNrf2, as confirmed through dual luciferase and qRT-PCR detection. Further, the enzyme activity tests demonstrated that McNrf2 could counteract Bap induced oxidative stress by inhibiting McSLC35E2. The current study provides valuable insights into the application of ChIP-seq technology in the research of marine mollusks, advancing our understanding of the key role of Nrf2 in antioxidant defense mechanisms, and highlighting the significance of SLC35E2 in the highly sophisticated regulation of oxidative stress response in marine invertebrates.
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