Biochar alleviates combined stress of ammonium and acids by firstly enriching Methanosaeta and then Methanosarcina
Biochar alleviates combined stress of ammonium and acids by firstly enriching Methanosaeta and then Methanosarcina
复制标题
生物炭首先富集甲烷菌，然后富集甲烷八叠球菌，缓解铵和酸的联合胁迫
DOI：
10.1016/j.watres.2015.12.029
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发表时间：
2016-03-01
期刊：
影响因子：
12.8
通讯作者：
He, Pinjing
中科院分区：
文献类型：
--
作者：
Lu, Fan;Luo, Chenghao;He, Pinjing
研究了不同粒径的生物炭对6g/L葡萄糖厌氧消化过程中氨氮(NH4+)抑制的缓解效果。与不添加生物炭的对照相比，添加2~5 mm、0.5~1 mm和75~150 mm生物炭的处理甲烷化滞后期分别减少23.9%、23.8%和5.9%，最大产甲烷速率分别提高47.1%、23.5%和44.1%。这些结果证实，在氨和酸的双重抑制风险下，生物炭加速了厌氧消化过程中甲烷化的启动。此外，细小的生物炭显著促进了挥发性脂肪酸(VFAS)的产生。对消化早期和后期以及悬浮态、生物炭松结态和生物炭紧密结合态的古生菌和细菌多样性的比较分析表明，在悬浮态组分中，产氢甲烷杆菌对氨氮具有积极的抗性。然而，乙酰破碎型甲烷藻可以通过提高对导电生物炭的亲和力而在VFas浓度高达60-80 mmoC/L的条件下存活，从而加速乙酸酯的降解。甲烷八叠球菌对生物炭紧密结合组分的定植改善了产甲烷的能力。选择合适的生物炭颗粒大小对于促进微生物细胞的初始定植很重要。(C)2015爱思唯尔有限公司。保留所有权利。
This investigation evaluated the effectiveness of biochar of different particle sizes in alleviating ammonium (NH4+) inhibition (up to 7 g-N/L) during anaerobic digestion of 6 g/L glucose. Compared to the control treatment without biochar addition, treatments that included biochar particles 2-5 mm, 0.5 -1 mm and 75-150 mu m in size reduced the methanization lag phase by 23.9%; 23.8% and 5.9%, respectively, and increased the maximum methane production rate by 47.1%, 23.5% and 44.1%, respectively. These results confirmed that biochar accelerated the initiation of methanization during anaerobic digestion under double inhibition risk from both ammonium and acids. Furthermore, fine biochar significantly promoted the production of volatile fatty acids (VFAs). Comparative analysis on the archaeal and bacterial diversity at the early and later stages of digestion, and in the suspended, biochar loosely bound, and biochar tightly bound fractions suggested that, in suspended fractions, hydrogenotrophic Methanobacterium was actively resistant to ammonium. However, acetoclastic Methanosaeta can survive at VFAs concentrations up to 60-80 mmol-C/L by improved affinity to conductive biochar, resulting in the accelerated initiation of acetate degradation. Improved methanogenesis was followed by the colonization of the biochar tightly bound fractions by Methanosarcina. The selection of appropriate biochar particles sizes was important in facilitating the initial colonization of microbial cells. (C) 2015 Elsevier Ltd. All rights reserved.