Tissue-Engineered Bone With 3-Dimensionally Printed β-Tricalcium Phosphate and Polycaprolactone Scaffolds and Early Implantation: An In Vivo Pilot Study in a Porcine Mandible Model
Tissue-Engineered Bone With 3-Dimensionally Printed β-Tricalcium Phosphate and Polycaprolactone Scaffolds and Early Implantation: An In Vivo Pilot Study in a Porcine Mandible Model
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DOI：
10.1016/j.joms.2015.01.021
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发表时间：
2015-05-01
影响因子：
1.9
通讯作者：
Troulis, Maria J.
中科院分区：
文献类型：
--
作者：
Konopnicki, Sandra;Sharaf, Basel;Troulis, Maria J.
目的：植入支架的深部骨渗透仍然是组织工程学中的一个挑战。本研究的目的是评价三维打印的β-磷酸三钙(β-TCP)和聚己内酯(PCL)支架在活体早期植入猪骨髓前体细胞(PBMPC)后的骨渗透深度。材料和方法：用50%的β-磷酸三钙和50%的PCL进行三维打印。收集、分离、扩增、分化为成骨细胞。将细胞种植到支架中，并在旋转透氧生物反应器系统中孵化14天。在每只小型猪(N=2只)的下颌骨上形成6个2×2 cm的缺损区。共有6个支架被放置在缺损区内，6个缺损区作为对照(未种植支架，n=3；空缺损区，n=3)。术后8周取材，行苏木精-伊红(H&E)、4‘，6-二氨基-2-苯基吲哚(DAPI)和CD31染色。分析包括标本中心的细胞计数、骨渗透和血管生成。结果：所有标本(N=12)的周围骨形成与天然骨相似。在6个支架中，有4个支架中央有骨形成。H&E染色切片的组织形态计量学分析显示，构建组的中央平均有22.1%的骨，而非种植的支架平均有1.87%的骨(P
Purpose: Deep bone penetration into implanted scaffolds remains a challenge in tissue engineering. The purpose of this study was to evaluate bone penetration depth within 3-dimensionally (3D) printed beta-tricalcium phosphate (beta-TCP) and polycaprolactone (PCL) scaffolds, seeded with porcine bone marrow progenitor cells (pBMPCs), and implanted early in vivo.Materials and Methods: Scaffolds were 3D printed with 50% beta-TCP and 50% PCL. The pBMPCs were harvested, isolated, expanded, and differentiated into osteoblasts. Cells were seeded into the scaffolds and constructs were incubated in a rotational oxygen-permeable bioreactor system for 14 days. Six 2- x 2-cm defects were created in eachmandible (N= 2 minipigs). In total, 6 constructs were placed within defects and 6 defects were used as controls (unseeded scaffolds, n = 3; empty defects, n = 3). Eight weeks after surgery, specimens were harvested and analyzed by hematoxylin and eosin (H&E), 4',6-diamidino-2-phenylindole (DAPI), and CD31 staining. Analysis included cell counts, bone penetration, and angiogenesis at the center of the specimens.Results: All specimens (N = 12) showed bone formation similar to native bone at the periphery. Of 6 constructs, 4 exhibited bone formation in the center. Histomorphometric analysis of the H& E-stained sections showed an average of 22.1% of bone in the center of the constructs group compared with 1.87% in the unseeded scaffolds (P