PATTERNS OF GENE-EXPRESSION AND SITES OF LATENCY IN HUMAN NERVE GANGLIA ARE DIFFERENT FOR VARICELLA-ZOSTER AND HERPES-SIMPLEX VIRUSES
PATTERNS OF GENE-EXPRESSION AND SITES OF LATENCY IN HUMAN NERVE GANGLIA ARE DIFFERENT FOR VARICELLA-ZOSTER AND HERPES-SIMPLEX VIRUSES
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DOI：
10.1073/pnas.85.24.9773
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
STRAUS, SE
中科院分区：
文献类型：
--
作者：
CROEN, KD;OSTROVE, JM;STRAUS, SE
用原位杂交技术研究了人感觉神经节急性和潜伏感染水痘-带状疱疹病毒(VZV)的细胞定位和病毒转录模式，并与潜伏感染的单纯疱疹病毒(HSV)进行了比较。将尸检获得的三叉神经节和背根神经节与与VZV或HSV片段同源的35S标记的单链RNA探针杂交。我们已经报道，单纯疱疹病毒持续存在于人类感觉神经元中，只表达一种转录产物家族，该家族与编码即刻早期蛋白的mRNA广泛重叠，但在极性上相反，称为感染细胞蛋白0(ICP0)。在目前的研究中，我们发现潜伏的VZV感染涉及非神经细胞，多个但不是全部的VZV基因被转录。相反，在水痘期间，神经细胞和非神经细胞都被感染，VZV基因组分析的所有区域都得到了表达。因此，人类神经节VZV和HSV感染的基因表达模式和细胞定位不同。这些差异可能是区分这些感染的临床特征的基础。
The cellular localization and viral transcription patterns of acute and latent varicella-zoster virus (VZV) infections of human sensory nerve ganglia were studied by in situ hybridization and compared with those of latent herpes simplex virus (HSV) infection. Trigeminal and dorsal root ganglia obtained at autopsy were hybridized with 35S-labeled single-stranded RNA probes homologous to VZV or HSV fragments. We have reported that HSV persists in human sensory neurons and expresses only one family of transcripts that overlap extensively with, but are opposite in polarity to, the mRNA encoding the immediate early protein termed infected cell protein 0 (ICP0). In the present study we find that latent VZV infection involves nonneuronal cells, and multiple, but not all, VZV genes are transcribed. In contrast, during varicella both neuronal and noneuronal cells are infected, with all regions of the VZV genome analyzed being expressed. Thus, the patterns of gene expression and cellular locations of VZV and HSV infections of human ganglia differ. The differences may underlie clinical features that distinguished these infections.