Surface modification of poly(dimethylsiloxane) microfluidic devices by ultraviolet polymer grafting
Surface modification of poly(dimethylsiloxane) microfluidic devices by ultraviolet polymer grafting
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DOI：
10.1021/ac025700w
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发表时间：
2002-08-15
影响因子：
7.4
通讯作者：
Allbritton, N
中科院分区：
文献类型：
--
作者：
Hu, SW;Ren, XQ;Allbritton, N
基于聚（二甲基硅氧烷）（PDMS）的微流体装置由于其易于制造和低成本而越来越受欢迎。尽管如此，仍然非常需要快速且容易地定制这些器件的表面特性的策略。我们展示了一个一步的程序共价连接聚合物的PDMS微通道表面的紫外接枝聚合。将丙烯酸、丙烯酰胺、二甲基丙烯酰胺、丙烯酸2-羟乙酯和聚乙二醇单甲氧基丙烯酸酯接枝到PDMS上以产生亲水表面。水滴在接枝表面的接触角低至45 °。与由天然PDMS组成的装置相比，由接枝PDMS构建的微通道容易填充水溶液。接枝的表面还显示出与氧化的PDMS相比，两种测试肽的吸附显著降低。接枝表面的微通道表现出的电渗迁移率的中间体所显示的原生和氧化的PDMS。与氧化PDMS的电渗流动性不同，接枝表面的电渗流动性在暴露于空气时保持稳定。两个测试肽在接枝微通道中的电泳分辨率相比，在由氧化的PDMS组成的微通道中有显着提高。通过使用适当的单体，应该可以使用UV接枝来赋予PDMS微流体装置各种表面性质。
Poly(dimethylsiloxane) (PDMS)-based microfluidic devices are increasing in popularity due to their ease of fabrication and low costs. Despite this, there is a tremendous need for strategies to rapidly and easily tailor the surface properties of these devices. We demonstrate a one-step procedure to covalently link polymers to the surface of PDMS microchannels by ultraviolet graft polymerization. Acrylic acid, acrylamide, dimediylactylamide, 2-hydroxylethyl acrylate, and poly(ethylene glycol)monomethoxyl acrylate were grafted onto PDMS to yield hydrophilic surfaces. Water droplets possessed contact angles as low as 45degrees on the grafted surfaces. Microchannels constructed from the grafted PDMS were readily filled with aqueous solutions in contrast to devices composed of native PDMS. The grafted surfaces also displayed a substantially reduced adsorption of two test peptides compared to that of oxidized PDMS. Microchannels with grafted surfaces exhibited electroosmotic mobilities intermediate to those displayed by native and oxidized PDMS. Unlike the electroosmotic mobility of oxidized PDMS, the electroosmotic mobility of the grafted surfaces remained stable upon exposure to air. The electrophoretic resolution of two test peptides in the grafted microchannels was considerably improved compared to that in microchannels composed of oxidized PDMS. By using the appropriate monomer, it should be possible to use UV grafting to impart a variety of surface properties to PDMS microfluidics devices.