Three‐dimensional numerical approach to investigate the substrate transport and conversion in an immobilized enzyme reactor
Three‐dimensional numerical approach to investigate the substrate transport and conversion in an immobilized enzyme reactor
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三维数值方法研究固定化酶反应器中的底物传输和转化
DOI：
10.1002/bit.10723
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发表时间：
2003
影响因子：
3.8
通讯作者：
A. Delgado
中科院分区：
文献类型：
--
作者：
S. Esterl;Özlem Özmutlu;C. Hartmann;A. Delgado
本文对固定化酶反应器中的动量和质量传递进行了数值研究。该模拟基于三维Navier-Stokes方程和标量输运方程的解，该输运方程具有用于输运和底物向产物转化的汇项。反应器由一个装有20个球形酶载体的容器组成。这些载体中的每一个都覆盖有活性酶层，在那里发生转化。为了解释生物化学活性，标量输运方程中的汇项由标准Michaelis-Menten方法表示。模拟给出了详细的信息，当地的基板和产品的浓度相对于外部和内部的运输限制。一个主要的焦点是设置在催化过程中的基板上的传输速度的影响。对于反应器性能分析，整体和局部输运过程由一组完整的无量纲变量描述。基质浓度，速度和效率的过程之间的相互作用可以研究与这些变量的帮助。不同的基板流入浓度的过程中的效果可以看出，在速度变化。该系统的流场表征使得有可能了解流体的机械性能及其对运输过程的重要性。通过空隙体积的流体运动的分布在反应器的不同部分具有不同的性质。这种现象对显著不同的质量传输区域的布置以及过程效率具有强烈的影响。利用给定的数据，还可以检测高、低和潜在酶活性的区域，并确定转化是否由于传质或反应阻力而受到限制。© 2003 Wiley Periodicals，Inc. Biotechnol Bioeng 83：780 - 789，2003.
This numerical study evaluates the momentum and mass transfer in an immobilized enzyme reactor. The simulation is based on the solution of the three‐dimensional Navier‐Stokes equation and a scalar transport equation with a sink term for the transport and the conversion of substrate to product. The reactor consists of a container filled with 20 spherical enzyme carriers. Each of these carriers is covered with an active enzyme layer where the conversion takes place. To account for the biochemical activity, the sink term in the scalar transport equation is represented by a standard Michaelis‐Menten approach. The simulation gives detailed information of the local substrate and product concentrations with respect to external and internal transport limitations. A major focus is set on the influence of the substrate transport velocity on the catalytic process. For reactor performance analysis the overall and the local transport processes are described by a complete set of dimensionless variables. The interaction between substrate concentration, velocity, and efficiency of the process can be studied with the help of these variables. The effect of different substrate inflow concentrations on the process can be seen in relation to velocity variations. The flow field characterization of the system makes it possible to understand fluid mechanical properties and its importance to transport processes. The distribution of fluid motion through the void volume has different properties in different parts of the reactor. This phenomenon has strong effects on the arrangement of significantly different mass transport areas as well as on process effectiveness. With the given data it is also possible to detect zones of high, low, and latent enzymatic activity and to determine whether the conversion is limited due to mass transfer or reaction resistances. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 780–789, 2003.