COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES
COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES
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DOI：
10.1038/168167a0
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发表时间：
1956-01-01
影响因子：
7.4
通讯作者：
SMITH, F
中科院分区：
文献类型：
--
作者：
DUBOIS, M;GILLES, KA;SMITH, F
用分配色层1，2分离后，用容量法测定糖的含量。根据我们的经验，这些方法不仅需要相当高的技巧，而且耗时长，对条件的微小变化很敏感。因此，我们试图开发一种简单的定量比色法。初步实验表明，在纯糖溶液中，菲试剂3和α-萘酚磺酸盐试剂效果很好，但在糖类的色谱分离中，只有丁醇、苯酚和丙酸等微量溶剂的存在使它们变得无用。
VOLUMETRIC procedures have been used for the quantitative determination of sugars after separation by partition chromatography1,2. It has been our experience that these methods not only require considerable skill, but also they are lengthy and sensitive to slight variation of the conditions. We have therefore attempted to develop a simple quantitative colorimetric procedure. Preliminary experiments showed that the anthrone3and the α-naphthol sulphonate4reagents give good results with pure sugar solutions, but the presence of only traces of solvents such as butanol, phenol and propionic acid used in the chromatographic separation of the sugars rendered them useless.