A microwell array device capable of measuring single-cell oxygen consumption rates.
A microwell array device capable of measuring single-cell oxygen consumption rates.
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DOI：
10.1016/j.snb.2008.10.036
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发表时间：
2009-01-15
影响因子：
8.4
通讯作者：
Lidstrom, Mary E.
中科院分区：
文献类型：
--
作者：
Molter, Timothy W.;McQuaide, Sarah C.;Suchorolski, Martin T.;Strovas, Tim J.;Burgess, Lloyd W.;Meldrum, Deirdre R.;Lidstrom, Mary E.
关键词：
由于对细胞群体异质性的兴趣，近年来用于研究单细胞的新技术和方法的开发急剧增加。理想的单细胞测量系统对于统计相关性来说将是高通量的，将测量最重要的细胞参数，并且最小化对正常细胞功能的破坏。我们开发了一种能够测量单细胞耗氧率（OCR）的微孔阵列装置。该OCR装置能够扩散分离单细胞，并且能够以非侵入性和相对高通量的方式以fmol/min分辨率定量测量单细胞消耗的氧。含有固定发光传感器的玻璃微孔阵列格式允许将来并入额外的细胞参数感测能力。为了证明OCR装置的实用性，我们确定了三种不同细胞系的一小组单细胞（12至18）的耗氧速率：鼠巨噬细胞系RAW 264.7、人上皮肺癌细胞系A549和人巴雷特食管细胞系CP-D。
Due to interest in cell population heterogeneity, the development of new technology and methodologies for studying single cells has dramatically increased in recent years. The ideal single cell measurement system would be high throughput for statistical relevance, would measure the most important cellular parameters, and minimize disruption of normal cell function. We have developed a microwell array device capable of measuring single cell oxygen consumption rates (OCR). This OCR device is able to diffusionally isolate single cells and enables the quantitative measurement of oxygen consumed by a single cell with fmol/min resolution in a non-invasive and relatively high throughput manner. A glass microwell array format containing fixed luminescent sensors allows for future incorporation of additional cellular parameter sensing capabilities. To demonstrate the utility of the OCR device, we determined the oxygen consumption rates of a small group of single cells (12 to 18) for three different cells lines: murine macrophage cell line RAW264.7, human epithelial lung cancer cell line A549, and human Barrett’s esophagus cell line CP-D.
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