Acidic peptide hydrogel scaffolds enhance calcium phosphate mineral turnover into bone tissue
Acidic peptide hydrogel scaffolds enhance calcium phosphate mineral turnover into bone tissue
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DOI：
10.1016/j.actbio.2012.04.003
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发表时间：
2012-07-01
影响因子：
9.7
通讯作者：
Rapaport, Hanna
中科院分区：
文献类型：
--
作者：
Amosi, Nadav;Zarzhitsky, Shlomo;Rapaport, Hanna
设计的多肽可以产生水凝胶形式的分子支架来支持组织再生。我们研究了含有富含天冬氨酸的-薄片形成肽的水凝胶和负载磷酸三钙（- tcp）的水凝胶对钙吸附和体外细胞培养以及体内骨再生的影响。水凝胶作为钙离子的有效储存库，在体外诱导成骨细胞分化。通过显微计算机断层扫描分析大鼠股骨远端骨缺损愈合的体内研究表明，与未处理的缺损相比，肽水凝胶本身诱导了更好的骨再生。用β - tcp负载的水凝胶处理骨缺损获得了更强的再生能力，表明肽水凝胶和矿物质协同作用增强骨再生。体内再生发现，水凝胶加载多孔β - tcp比水凝胶加载非多孔矿物更好。综上所述，生物相容性和可生物降解的基质可以有效地促进β - tcp转化为骨矿物质，而这些基质富含阴离子部分，可以有效地吸附钙离子，同时支持细胞成骨活性。(C) 2012材料学报Elsevier Ltd.出版。版权所有。
Designed peptides may generate molecular scaffolds in the form of hydrogels to support tissue regeneration. We studied the effect of hydrogels comprising beta-sheet-forming peptides rich in aspartic amino acids and of tricalcium phosphate (beta-TCP)-loaded hydrogels on calcium adsorption and cell culture in vitro, and on bone regeneration in vivo. The hydrogels were found to act as efficient depots for calcium ions, and to induce osteoblast differentiation in vitro. In vivo studies on bone defect healing in rat distal femurs analyzed by microcomputerized tomography showed that the peptide hydrogel itself induced better bone regeneration in comparison to non-treated defects. A stronger regeneration capacity was obtained in bone defects treated with beta-TCP-loaded hydrogels, indicating that the peptide hydrogels and the mineral act synergistically to enhance bone regeneration. In vivo regeneration was found to be better with hydrogels loaded with porous beta-TCP than with hydrogels loaded with non-porous mineral. It is concluded that biocompatible and biodegradable matrices, rich in anionic moieties that efficiently adsorb calcium ions while supporting cellular osteogenic activity, may efficiently promote beta-TCP turnover into bone mineral. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.