Possible role of human foamy virus in Graves' disease.
Possible role of human foamy virus in Graves' disease.
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DOI：
10.1159/000150300
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发表时间：
1993
期刊：
影响因子：
4.6
通讯作者：
Georg Wick;K. Trieb;Adriano Aguzzi;Heidrun Recheis;Hans Anderl;Beatrix Grubeck-Loebenstein
中科院分区：
文献类型：
--
作者：
Georg Wick;K. Trieb;Adriano Aguzzi;Heidrun Recheis;Hans Anderl;Beatrix Grubeck-Loebenstein
人泡沫病毒（HFV）是spumaretrovirae逆转录病毒家族的一员。除了三种逆转录病毒结构基因，gag， pol和env， HFV还含有被称为bel的调控序列。泡沫病毒以前与人类甲状腺疾病有关，特别是DeQuervain甲状腺炎。在间接免疫荧光试验中，我们证实了7/7 Graves病患者的甲状腺反应性和HFV gag蛋白抗体。与pol、env和bel蛋白抗体无反应性。结节实质瘤9例，滤泡癌4例，正常甲状腺2例，甲状腺全阴性。5例桥本病甲状腺4例阴性，1例单小灶抗gag抗体反应。均匀的免疫荧光染色仅限于甲状腺上皮细胞之间的基底和外侧细胞间区。将这些研究扩展到1例Graves病合并恶性眼球突出的患者球后组织，发现成纤维细胞和脂肪细胞的抗gag抗体阳性，但眼肌未见阳性。此外，我们成功地从该患者的球后组织中建立了几种t细胞系。它们是CD8+，与外周血细胞相比，与自体眶后成纤维细胞共培养后增殖。这些观察结果是否与格雷夫斯病的发病机制有关还有待确定。
Human foamy virus (HFV) is a member of the retroviral family of Spumaretrovirinae. In addition to the three retroviral structural genes, gag, pol and env, HFV also contains regulatory sequences, called bel. Foamy viruses have been previously associated with human thyroid disease, notably DeQuervain's thyroiditis. In indirect immunofluorescence tests we have demonstrated the reactivity of the thyroid glands of 7/7 patients with Graves' disease and antibodies to HFV gag proteins. No reactivity was observed with antibodies to pol, env and bel proteins. Nine thyroids of patients with struma parenchymatosa, 4 with follicular carcinoma and 2 normal thyroids were negative throughout. From the thyroids of 5 patients with Hashimoto's disease, 4 were negative and 1 showed a single small focus of anti-gag antibody reactivity. The uniform immunofluorescent staining was restricted to the basal and lateral intercellular areas between the thyroid epithelial cells. Extension of these studies to the retrobulbar tissue of 1 Graves' disease patient with malignant exophthalmus revealed positive staining with anti-gag antibodies of fibroblasts and fat cells but not eye muscles. Furthermore, we were successful in establishing several T-cell lines derived from the retrobulbar tissue of this patient. They were CD8+ and proliferated, in contrast to peripheral blood cells, upon cocultivation with autologous retroorbital fibroblasts. It remains to be determined whether these observations are of relevance in the pathogenesis of Graves' disease.