PHOSPHORYLATION OF FAR1 IN RESPONSE TO ALPHA-FACTOR - A POSSIBLE REQUIREMENT FOR CELL-CYCLE ARREST
PHOSPHORYLATION OF FAR1 IN RESPONSE TO ALPHA-FACTOR - A POSSIBLE REQUIREMENT FOR CELL-CYCLE ARREST
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DOI：
10.1091/mbc.3.4.445
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发表时间：
1992-04-01
影响因子：
3.3
通讯作者：
HERSKOWITZ, I
中科院分区：
文献类型：
--
作者：
CHANG, F;HERSKOWITZ, I
酵母a细胞暴露于α因子导致细胞停滞在细胞周期的G1期。FAR 1基因是这种细胞周期阻滞所必需的;其产物是抑制G1周期蛋白CLN 2所必需的。早期的工作表明，α-因子导致FAR 1转录增加数倍于可测量的基础水平。我们现在表明，转录诱导FAR 1从异源启动子是不足以抑制CLN 2的α-因子的情况下。我们还表明，FAR 1是磷酸化的α-因子的反应，并提出这种磷酸化可能需要FAR 1的活动。
Exposure of yeast a cells to alpha-factor causes cells to arrest in the G1 phase of the cell cycle. The FAR1 gene is required for this cell-cycle arrest; its product is necessary for the inhibition of a G1 cyclin, CLN2. Earlier work demonstrated that alpha-factor caused an increase in the transcription of FAR1 severalfold over a measurable basal level. We now show that transcriptional induction of FAR1 from a heterologous promoter is not sufficient to inhibit CLN2 in the absence of alpha-factor. We also show that FAR1 is phosphorylated in response to alpha-factor and propose that this phosphorylation may be required for FAR1 activity.