Predominant expression of alternative PAX3 and PAX7 forms in myogenic and neural tumor cell lines.
Predominant expression of alternative PAX3 and PAX7 forms in myogenic and neural tumor cell lines.
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发表时间：
1999-11
期刊：
影响因子：
11.2
通讯作者：
F. Barr;J. Fitzgerald;J. Ginsberg;Michelle L. Vanella;Richard J. Davis;J. Bennicelli
中科院分区：
文献类型：
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作者：
F. Barr;J. Fitzgerald;J. Ginsberg;Michelle L. Vanella;Richard J. Davis;J. Bennicelli
PAX 3和PAX 7是在早期神经和肌源性发育期间表达的密切相关的配对盒家族成员。PAX 3和PAX 7 mRNA在胚胎性横纹肌肉瘤、神经母细胞瘤、尤文氏肉瘤和黑色素瘤细胞系中的表达分析揭示了与相应胚胎谱系相似的肿瘤特异性表达模式。虽然哺乳动物PAX 3和PAX 7基因被报道含有8个外显子，但我们发现这些肿瘤细胞系中主要的PAX 3和PAX 7转录物含有先前未表征的第9个外显子。这些剪接事件改变了编码产物的COOH末端编码区，但不改变转录活性，如使用具有PAX 3/PAX 7结合位点模型的报告基因所测定的。然而，在鸟类和鱼类基因组的相应基因内几乎相同的COOH末端区域的发现表明这些区域的保守功能作用需要进一步研究。
PAX3 and PAX7 are closely related paired box family members expressed during early neural and myogenic development. Assay of PAX3 and PAX7 mRNA expression in embryonal rhabdomyosarcoma, neuroblastoma, Ewing's sarcoma, and melanoma cell lines revealed tumor-specific expression patterns similar to the corresponding embryonic lineages. Although the mammalian PAX3 and PAX7 genes were reported to contain eight exons, we found that the predominant PAX3 and PAX7 transcripts in these tumor lines contain previously uncharacterized ninth exons. These splicing events alter the COOH-terminal coding regions of the encoded products but do not alter the transcriptional activity as assayed using a reporter gene with a model PAX3/PAX7 binding site. However, the findings of nearly identical COOH-terminal regions within the corresponding genes of the avian and fish genomes suggest conserved functional roles for these regions that require further investigation.