Enhanced phagocytosis of CD47-deficient red blood cells by splenic macrophages requires SHPS-1
Enhanced phagocytosis of CD47-deficient red blood cells by splenic macrophages requires SHPS-1
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DOI：
10.1016/j.bbrc.2006.03.094
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发表时间：
2006-05-19
影响因子：
3.1
通讯作者：
Matozaki, T
中科院分区：
文献类型：
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作者：
Ishikawa-Sekigami, T;Kaneko, Y;Matozaki, T
红细胞（RBC）上的CD 47与巨噬细胞上的SHPS-1的相互作用涉及阻止后者细胞对前者细胞的吞噬作用。事实上，与野生型RBC相比，输注的CD 47缺陷型（CD 47（-/-））RBC从野生型小鼠血流中的清除率显著增加。相反，输注野生型RBC的清除率在表达缺乏大部分蛋白质胞质区域的SHPS-1突变形式的小鼠中显著增加。然而，我们在此发现，SHPS-1突变小鼠中CD 47（-/-）RBC的清除率极低。此外，与野生型巨噬细胞相比，SHPS-1突变小鼠脾巨噬细胞对CD 47（-/-）RBC的吞噬作用显著降低。因此，这些结果表明，红细胞上的CD 47在巨噬细胞吞噬作用的负调节和循环红细胞寿命的测定中具有额外的作用。(c)2006年爱思唯尔公司All rights reserved.
The interaction of CD47 on red blood cells (RBCs) with SHPS-1 on macrophages is implicated to prevent the phagocytosis of the former cells by the latter cells. Indeed, the rate of clearance of transfused CD47-deficient (CD47(-/-)) RBCs from the bloodstream of wild-type mice was markedly increased compared with wild-type RBCs. Conversely, the rate of clearance of transfused wild-type RBCs was markedly increased in mice that expressed a mutant form of SHPS-1 lacking most of the cytoplasmic region of the protein. However, we here found that the clearance of CD47(-/-) RBCs in SHPS-1 mutant mice was minimal. In addition, the phagocytosis of CD47(-/-) RBCs by splenic macrophages from SHPS-1 mutant mice was markedly reduced compared with wild-type macrophages. These results thus suggest an additional role for CD47 on RBCs in the negative regulation of phagocytosis by macrophages and in determination of the life span of circulating RBCs. (c) 2006 Elsevier Inc. All rights reserved.