GPI-anchored receptor clusters transiently recruit Lyn and G alpha for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1.
GPI-anchored receptor clusters transiently recruit Lyn and G alpha for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1.
复制标题
GPI锚定的受体簇瞬时募集lyn和g alpha进行临时簇固定和LYN激活：单分子跟踪研究1。
DOI：
10.1083/jcb.200609174
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发表时间：
2007-05-21
影响因子：
7.8
通讯作者：
Kusumi, Akihiro
中科院分区：
文献类型：
--
作者：
Suzuki, Kenichi G N;Fujiwara, Takahiro K;Sanematsu, Fumiyuki;Iino, Ryota;Edidin, Michael;Kusumi, Akihiro
糖基磷脂酰肌醇锚定受体（GPI-ARs）的信号转导机制已通过跟踪活细胞中的单分子进行了研究。在生理水平的CD 59（和其他GPI-AR）的接合或胶体金诱导的交联后，形成含有3至9个CD 59分子的CD 59簇，并且单个Gαi2或林恩分子（GFP缀合物）通过蛋白质-蛋白质和脂质-脂质（筏）相互作用表现出频繁但短暂（分别为133和200 ms）的CD 59簇募集。每个CD 59簇经历肌动蛋白依赖性暂时固定（0.57秒寿命;刺激诱导的侧向扩散暂时停滞[STALL]，诱导IP 3产生）和缓慢扩散（1.2秒）的交替期。在Gαi2募集后立即诱导CD 59簇的STALL。因为Gαi2和林恩都是STALL所需的，并且因为林恩组成性地募集到CD 59簇，所以CD 59簇的STALL可能是由Gαi2与相同CD 59簇内的林恩结合并随后激活所诱导的。
The signaling mechanisms for glycosylphosphatidylinositol-anchored receptors (GPI-ARs) have been investigated by tracking single molecules in living cells. Upon the engagement or colloidal gold–induced cross-linking of CD59 (and other GPI-ARs) at physiological levels, CD59 clusters containing three to nine CD59 molecules were formed, and single molecules of Gαi2 or Lyn (GFP conjugates) exhibited the frequent but transient (133 and 200 ms, respectively) recruitment to CD59 clusters, via both protein–protein and lipid–lipid (raft) interactions. Each CD59 cluster undergoes alternating periods of actin-dependent temporary immobilization (0.57-s lifetime; stimulation-induced temporary arrest of lateral diffusion [STALL], inducing IP3 production) and slow diffusion (1.2 s). STALL of a CD59 cluster was induced right after the recruitment of Gαi2. Because both Gαi2 and Lyn are required for the STALL, and because Lyn is constitutively recruited to CD59 clusters, the STALL of CD59 clusters is likely induced by the Gαi2 binding to, and its subsequent activation of, Lyn within the same CD59 cluster.