Enhanced thromboxane synthesis during chronic reductions in uterine perfusion pressure in pregnant rats.
Enhanced thromboxane synthesis during chronic reductions in uterine perfusion pressure in pregnant rats.
复制标题
妊娠大鼠子宫灌注压长期降低期间血栓素合成增强。
DOI：
10.1016/s0895-7061(02)02975-8
复制
发表时间：
2002
影响因子：
3.2
通讯作者：
Granger,JoeyP
中科院分区：
文献类型：
--
作者：
Llinás,MariaT;Alexander,BarbaraT;Seedek,Mona;Abram,SeanR;Crell,Anna;Granger,JoeyP
本研究的目的是确定血栓素A2（TXA 2）在清醒的、慢性仪器化的妊娠高血压（PIH）大鼠模型中的作用，该模型由子宫灌注压（RUPP）的慢性降低产生。有效肾血浆流量（ERPF）和24小时尿TXB 2排泄结果在妊娠20 d时，与正常妊娠对照组相比，RUPP大鼠的MAP（125 ± 3 mm Hg/100 ± 2 mm Hg）显著升高（P<0.05）。RUPP组血压升高时尿TXB_2浓度显著高于正常妊娠组（3663 ± 488 v2646 ± 257 pg/24 h）（P<0.05）。与妊娠对照组相比，RUPP大鼠的基线GFR（分别为1.74 ± 0.13和2.40 ± 0.20 mL/min，P<0.05）和ERPF（分别为5.13 ± 0.44和6.44 ± 0.58 mL/min）降低。输注TX受体拮抗剂SQ 29，548（2 mg/kg推注加2 mg/kg/h输注）对RUPP妊娠大鼠的MAP增加无显著影响。同样，ERPF和GFR并没有改变在急性阻塞TXA 2 receptor in this group.ConclusionThese研究结果表明，TXA 2的生产增强不发挥重要作用，在调解高血压和肾血管收缩所产生的慢性RUPP在怀孕的大鼠。
BackgroundThe purpose of this study was to determine the role of thromboxane A2(TXA2) in a conscious, chronically instrumented rat model of pregnancy-induced hypertension (PIH) produced by chronic reductions in uterine perfusion pressure (RUPP).MethodsMean arterial pressure (MAP), glomerular filtration rate (GFR), effective renal plasma flow (ERPF) and 24-h urinary excretion of TXB2(metabolite of TXA2) were determined in normal pregnant rats and RUPP pregnant rats.ResultsAt day 20 of pregnancy, RUPP rats showed a significantly (P< .05) higher MAP (125 ± 3 mm Hgv100 ± 2 mm Hg) as compared with normal pregnant controls. The elevation in arterial pressure in RUPP group was associated with a marked increase (P< .05) in the urinary concentration of TXB2compared with normal pregnant group (3663 ± 488v2646 ± 257 pg/24 h). Baseline GFR (1.74 ± 0.13v2.40 ± 0.20 mL/min, respectively,P< .05) and ERPF (5.13 ± 0.44v6.44 ± 0.58 mL/min, respectively) were decreased in RUPP rats relative to pregnant controls. Infusion of a TX receptor antagonist, SQ 29,548 (2 mg/kg bolus plus 2 mg/kg per h infusion) had no significant effect on increased MAP in RUPP pregnant rats. Similarly, ERPF and GFR did not change during acute blockade of TXA2receptors in this group.ConclusionThese findings suggest that enhanced production of TXA2does not play a major role in mediating the hypertension and renal vasoconstriction produced by chronic RUPP in pregnant rats.