Direct effect of testosterone and its 5alpha-reduced metabolites on pituitary LH and FSH release in vitro: change in pituitary responsiveness to hypothalamic extract.
Direct effect of testosterone and its 5alpha-reduced metabolites on pituitary LH and FSH release in vitro: change in pituitary responsiveness to hypothalamic extract.
复制标题
睾酮及其 5α 还原代谢物对体外垂体 LH 和 FSH 释放的直接影响：垂体对下丘脑提取物反应性的变化。
DOI：
10.1210/endo-96-2-253
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发表时间：
1975
期刊：
影响因子：
4.8
通讯作者：
J. Weisz
中科院分区：
文献类型：
--
作者：
L. Kao;J. Weisz
采用连续流式孵育（灌注）系统检测睾酮(T)及其3种5 α还原代谢物双氢睾酮（DHT）、5 α -雄甾烷-3 α、17 β -二醇（3 α -二醇）及其3 β -表二醇（3 β -二醇）对下丘脑提取物（HE）诱导的LH和FSH释放的影响。在没有类固醇的情况下，连续相同的HE脉冲，每次持续10分钟，在8小时内每隔一小时给药，引起高度可重复的LH和FSH释放。在实验灌注中，在连续输注类固醇4-6小时期间，每隔一小时给药的标准10分钟HE脉冲释放的LH和FSH量与同一垂体对类固醇输注开始前和停止后给予的标准HE测试脉冲的反应进行比较。所有测试的雄激素都改变了垂体反应。在0.1杯/毫升和1.0杯/毫升剂量水平下，两种类固醇对垂体反应性的影响是不同的。它们在这两种剂量下的作用可分为三类，取决于最初是否有：1)HE诱导的LH释放（T和3 -二醇）增加，2)FSH和LH释放（DHT）增加，或3)促性腺激素（3 -二醇）释放没有增加。所有雄激素最终都抑制了垂体对HE的反应，并且都与LH和FSH释放比例的变化有关。当T和3 - β -二醇的剂量增加到10马克/毫升或DHT的剂量降低到0.01马克/毫升时，未见初始刺激阶段。表甾酮，生物活性的T的表聚体，没有改变脑垂体的反应性。
A continuous flow incubation (perifusion) system was used to examine the effect of testosterone (T) and three of its 5alpha-reduced metabolites, dihydrotestosterone (DHT), 5alpha-androstane-3alpha, 17beta-diol (3alpha-Adiol) and its 3beta-epimer (3beta-Adiol) on LH and FSH release, induced by hypothalamic extract (HE). In the absence of steroids, successive identical pulses of HE, of 10 min duration each, administered at hourly intervals over a 8-hr period, caused highly reproducible release of LH and FSH. In experimental perifusions, the amounts of LH and FSH released in response to standard 10-min pulses of HE administered at hourly intervals during the continuous infusion of steroid for 4-6 hr were compared with the responses of the same pituitaries to the standard test pulses of HE given before the start of the steroid infusion and after its cessation. All the androgens tested altered pituitary responsiveness. At the 0.1 and 1.0 mug/ml dose level there were differences between the steroids in the way they influenced the responsiveness of the pituitary overtime. Their effects at these two doses fell into three categories depending on whether there was initially: 1) an augmentation of HE induced LH release (T and 3beta-Adiol), 2) augmentation of both FSH and LH release (DHT), OR 3) NO AUGMENTATION IN THE RELEASE OF EITHer gonadotrophin (3alpha-Adiol). All the androgens ultimately suppressed pituitary responsiveness to HE and all were associated with changes in the ratios of LH and FSH released. When the dose of T and 3 beta-Adiol was raised to 10 mug/ml or that of DHT lowered to 0.01 mug/ml the initial stimulatory phase was not seen. Epitestosterone, the biologically inactive epimer of T, did not alter the responsiveness of the pituitary of HE.