Studies on the kinetics of expression of cell cycle dependent proto-oncogenes during mitogen-induced liver cell proliferation.
Studies on the kinetics of expression of cell cycle dependent proto-oncogenes during mitogen-induced liver cell proliferation.
复制标题
丝裂原诱导的肝细胞增殖过程中细胞周期依赖性原癌基因表达动力学的研究。
DOI：
10.1016/0304-3835(89)90186-9
复制
发表时间：
1989
期刊：
影响因子：
9.7
通讯作者：
Sarma,DS
中科院分区：
文献类型：
--
作者：
Coni,P;Bignone,FA;Pichiri,G;Ledda-Columbano,GM;Columbano,A;Rao,PM;Rajalakshmi,S;Sarma,DS
本研究旨在确定雄性 Wistar 大鼠肝脏中 DNA 合成和细胞周期依赖性原癌基因表达对两种类型细胞增殖刺激的反应动力学。 DNA 合成的峰值出现在 2·3 部分肝切除诱导的代偿性细胞增殖刺激后约 24 小时，以及用单剂量硝酸铅（10 μmol 100 g 体重，通过股静脉）获得有丝分裂刺激后约 36 小时。尽管两种增殖刺激均诱导c-fos、c-myc和c-Ha-ras的表达，但在丝裂原诱导的细胞增殖中，c-fos表达增加的程度要低4至5倍。此外，虽然c-myc的表达在部分肝切除后4小时恢复到基础水平，但在硝酸铅诱导的肝细胞增殖过程中，c-myc的诱导表达持续长达40小时。
The present study was undertaken to determine the kinetics of DNA synthesis and expression of cell cycle dependent protooncogenes in response to two types of cell proliferative stimuli in male Wistar rat liver. The peak of DNA synthesis was approximately 24 h after a compensatory cell proliferative stimulus induced by 2 3 partial hepatectomy and approximately 36 h following a mitogenic stimulus obtained with a single dose of lead nitrate (10 μmol 100 g body wt, through femoral vein). Even though both proliferative stimuli induced the expression of c-fos, c-myc and c-Ha-ras, the extent of the increase in c-fos expression was 4-to 5-fold less in mitogen-induced cell proliferation. In addition, while the expression of c-myc, following partial hepatectomy returned to basal level by 4 h, the induced expression of c-myc persisted for up to 40 h during the lead nitrate-induced liver cell proliferation.