Deoxyribonucleic Acid Base Sequence Studies on Glucan- Producing and Glucan-Negative Strains of Streptococcus mitior
Deoxyribonucleic Acid Base Sequence Studies on Glucan- Producing and Glucan-Negative Strains of Streptococcus mitior
复制标题
轻度链球菌产葡聚糖和葡聚糖阴性菌株的脱氧核糖核酸碱基序列研究
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发表时间：
1978
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通讯作者：
Allan J. Munzenmaier
中科院分区：
文献类型：
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作者：
A. Coykendall;Allan J. Munzenmaier
用杂交法比较了14株缓解链球菌的脱氧核糖核酸序列相似性。有些菌株能产生葡聚糖。脱氧核糖核酸在氯化铯中用常规方法和离心法进行提纯。在含有S1核酸酶的溶液中和膜过滤器上进行杂交。结果显示，两组菌株具有中等同源性。产生葡聚糖的菌株代表了包括一些葡聚糖阴性菌株在内的一个群。S1法的杂交结果比Filter法少，说明两组间存在一定的序列差异。两组鸟嘌呤+胞嘧啶含量略有差异。
The deoxyribonucleic acid base sequence similarities among 14 strains of Streptococcus mitior were assessed by hybridization. Some strains produce glucan. Deoxyribonucleic acid was purified by both a conventional method and by centrifugation in CsCl. Hybridizations were done in solution with S1 nuclease and on membrane filters. The results showed two groups of moderately homologous strains. The glucan-producing strains represented a group which included some glucan-negative strains. Hybridization by the S1 method was less than with the filter method, indicating some sequence divergence within the two groups. There was a slight difference in guanine-plus-cytosine contents of the two groups.