Sulfated Astragalus polysaccharide can regulate the inflammatory reaction induced by LPS in Caco2 cells
Sulfated Astragalus polysaccharide can regulate the inflammatory reaction induced by LPS in Caco2 cells
复制标题
硫酸黄芪多糖调节LPS诱导的Caco2细胞炎症反应
DOI：
10.1016/j.ijbiomac.2013.05.037
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发表时间：
2013-09-01
影响因子：
8.2
通讯作者：
Yao, Junhu
中科院分区：
文献类型：
--
作者：
Wang, Xiaofei;Wang, Siyu;Yao, Junhu
本研究评价硫酸黄芪多糖（SAPS）对LPS诱导的Caco2细胞炎症反应的影响。采用氯磺酸-吡啶法进行了磺化改性。用25、50、100 μ g/mL SAPS或100 μ g/mL黄芪多糖（Astragalus多糖，APS）培养Caco2细胞24h。然后，添加1 μ g/mL LPS，持续24 h，以触发炎症反应。以DMEM培养基作为空白对照。本研究中，LPS刺激显著提高了tnf - α、IL-1 β、IL-8和TLR4 mRNA的表达，降低了ZO-1和occludin的表达。与LPS对照组相比，黄芪多糖（100 μ g/mL）或黄芪多糖（100 μ g/mL）均降低了tnf - α、IL-1 β和IL-8的表达。此外，25 μ g/mL和50 μ g/mL SAPS可下调tnf - α。IL-1 β的表达。黄芪多糖（100 μ g/mL）上调occludin表达，但不影响ZO-1表达。低剂量SAPS （25 μ g/mL和50 μ g/mL）可上调ZO-1和occludin的表达。与其他组相比，SAPS组在所有SAPS浓度下TLR4的表达均较低。这些结果表明，SAPS是比APS更有效的体外抗炎剂。(C) 2013 Elsevier B.V.版权所有
This study evaluates the effect of sulfated Astragalus polysaccharide (SAPS) on inflammatory reaction induced by LPS in Caco2 cells. Sulfated modification was conducted using the chlorosulfonic acid-pyridine method. Caco2 cells were cultured with 25, 50 and 100 mu g/mL SAPS or 100 mu g/mL Astragalus polysaccharide (APS) for 24h. Then, 1 mu g/mL LPS was added for the next 24 h to trigger an inflammatory response. DMEM culture medium was used as a blank control. In present study, LPS stimulation significantly increased the mRNA expression of TNF-alpha, IL-1 beta, IL-8 and TLR4, and reduced the expression of ZO-1 and occludin. Compared with the LPS control group, APS (100 mu g/mL) or SAPS (100 mu g/mL) administration decreased the expression of TNF-alpha, IL-1 beta and IL-8. Moreover, 25 mu g/mL and 50 mu g/mL SAPS down-regulated TNF-alpha. and IL-1 beta expression. APS administration (100 mu g/mL) up-regulated occludin expression, but did not affect ZO-1 expression. However, the expression of ZO-1 and occludin was up-regulated by lower dose SAPS administration (25 mu g/mL and 50 mu g/mL). Compared with the other groups, the expression of TLR4 was lower in the SAPS group at all concentrations of SAPS. These results suggest that SAPS was to be a more effective anti-inflammatory agent than APS in vitro. (C) 2013 Elsevier B.V. All rights reserved.