A Low Shear Stress Modular Bioreactor for Connected Cell Culture Under High Flow Rates
A Low Shear Stress Modular Bioreactor for Connected Cell Culture Under High Flow Rates
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DOI：
10.1002/bit.22671
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发表时间：
2010-05-01
影响因子：
3.8
通讯作者：
Ahluwalia, A.
中科院分区：
文献类型：
--
作者：
Mazzei, D.;Guzzardi, M. A.;Ahluwalia, A.
描述了一种通用的“板上系统”模块化多隔室生物反应器阵列，其使得微孔方案能够直接转移到生物反应器模块，而无需重新设计细胞培养实验或方案。模块化生物反应器易于组装和使用，并且可以很容易地与标准对照进行比较，因为细胞数量和培养基体积非常相似。从流体动力学和质量传输的考虑，模块化的生物反应器室首先建模，然后使用“毫模塑”，一种技术，从软光刻适应制造。在确认系统中的剪切应力在可用流速范围内极低后，使用肝细胞检测生物反应器室。结果表明，当流速低于500 μ L/min时，对应于细胞培养表面处10（-5）Pa或更小的壁剪切应力，生物反应器室可以增加或维持细胞活力和功能。Biotechnol. Bioeng. 2010;106：127-137. (C)2010 Wiley Periodicals，Inc.
A generic "system on a plate" modular multi-compartmental bioreactor array which enables microwell protocols to be transferred directly to the bioreactor modules, without redesign of cell culture experiments or protocols is described. The modular bioreactors are simple to assemble and use and can be easily compared with standard controls since cell numbers and medium volumes are quite similar. Starting from fluid dynamic and mass transport considerations, a modular bioreactor chamber was first modeled and then fabricated using "milli-molding," a technique adapted from soft lithography. After confirming that the shear stress was extremely low in the system in the range of useful flow rates, the bioreactor chambers were tested using hepatocytes. The results show that the bioreactor chambers can increase or maintain cell viability and function when the flow rates are below 500 mu L/min, corresponding to wall shear stresses of 10(-5) Pa or less at the cell culture surface. Biotechnol. Bioeng. 2010;106: 127-137. (C) 2010 Wiley Periodicals, Inc.